Mouse MANF Gene cDNA Clone / ORF Clone

MANF cDNA Clone Datasheet

MANF cDNA Clone Product Information

• Gene Bank Ref.ID: NM_029103.3
• cDNA Size: 540
• Gene Synonym: Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik; Manf
• Source: Mouse

MANF cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mesencephalic astrocyte-derived neurotrophic factor DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 2682 C/T not causing the amino acid variation.

MANF cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MANF cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MANF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGGCTA CGCGCGGGCT GGCGGTAGCG CTGGCCCTGA GCGTGCTGCC TGACAGCCGG
61       GCGCTGCGGC CAGGAGACTG TGAAGTTTGT ATTTCTTATC TGGGACGATT TTACCAGGAC
121      CTCAAAGACA GAGATGTCAC ATTTTCACCA GCCACTATTG AAGAAGAACT TATAAAGTTT
181      TGCCGTGAAG CAAGAGGCAA AGAGAATCGG TTGTGCTACT ACATTGGAGC CACAGATGAT
241      GCTGCCACCA AGATCATCAA TGAGGTGTCG AAGCCCCTGG CCCACCATAT CCCTGTGGAA
301      AAGATCTGTG AGAAGCTGAA GAAGAAAGAC AGCCAGATCT GTGAACTAAA ATACGACAAG
361      CAGATTGACC TGAGCACAGT GGACCTGAAG AAGCTCCGGG TGAAAGAGCT GAAGAAGATC
421      CTGGACGACT GGGGGGAGAT GTGCAAAGGC TGTGCAGAAA AGTCTGACTA TATCCGGAAG
481      ATAAATGAAC TGATGCCTAA ATACGCCCCC AAGGCAGCCA GCGCACGGAC TGATCTGTAG

 

Quality Control:cDNA sequencing results is available upon request.