Anti-ARMET Antibody

Cat: 11324-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All ARMET Reagents
CRO Services
Product recommended

All ARMET Reagents
Anti-ARMET Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ARMET Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human ARMET
Immunogen
Recombinant Human MANF / ARMET protein (Catalog#11324-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human MANF / ARMET (rh MANF / ARMET; Catalog#11324-H08H; NP_006001.3; Met 1-Leu 179). MANF / ARMET specific IgG was purified by Human MANF / ARMET affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ARMET Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ARMET Antibody (Rabbit Polyclonal antibody) Images
Human MANF Western blot (WB) 13393

Anti-MANF rabbit polyclonal antibody at 1:500 dilution

Lane A: 293 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:17 kDa

Observed band size:19 kDa

Human MANF Immunoprecipitation(IP) 17689

MANF was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-MANF rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MANF rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size: 15 kDa

Anti-ARMET Antibody Alternative Names
Anti-ARMET Antibody;Anti-ARP Antibody
ARMET Background Information

Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF and ARMET, is a secreted protein which belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types.

Full Name
mesencephalic astrocyte-derived neurotrophic factor
References
  • Shridhar V., et al., 1996, Oncogene 12:1931-1939.
  • Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
  • Petrova P., et al., 2003, J. Mol. Neurosci. 20:173-188.
  • Lindholm, P. et al., 2008, Mol Cell Neurosci. 39 (3):356-71.
  • Airavaara, M. et al., 2010, Exp Neurol. 225 (1):104-13.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ARMET Antibody Rabbit Polyclonal ELISA 50954-T16
    Anti-ARMET Antibody Rabbit Monoclonal ELISA 50954-R012
    Anti-ARMET Antibody Rabbit Polyclonal ELISA 50954-RP01
    Anti-ARMET Antibody Rabbit Polyclonal WB,IP 11324-T36
    info info
    添加购物车成功! 添加购物车失败!