MAG / GMA Protein

Myelin associated glycoprotein Protein Datasheet

MAG / GMA Protein Product Information

• Synonym: MAG, GMA
• Protein Construction: A DNA sequence encoding the human MAG (P20916-1) (Met1-Pro516) was expressed with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

MAG / GMA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 20
• Molecular Mass: The recombinant human MAG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 738 amino acids and has a predicted molecular mass of 81.7 kDa. The apparent molecular mass of the protein is approximately 113 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MAG / GMA protein

MAG / GMA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MAG / GMA Protein Description

The myelin-associated glycoprotein (MAG) is a transmembrane glycoprotein of 100kDa localized in myelin sheaths of periaxonal Schwann cell and oligodendroglial membranes where it functions in glia-axon interactions. MAG contains five immunoglobulin-like domains and belongs to the sialic-acid-binding subgroup of the Ig superfamily. It appears to function both as a receptor for an axonal signal that promotes the differentiation, maintenance and survival of oligodendrocytes and as a ligand for an axonal receptor that is needed for the maintence of myelinated axons. MAG contains a carbohydrate epitope shared with other glycoconjugates that is a target antigen in autoimmune peripheral neuropathy associated with IgM gammopathy and has been implicated in a dying back oligodendrogliopathy in multiple sclerosis. MAG is considered as a transmembrane protein of both CNS and PNS myelin and it strongly inhibits neurite outgrowth in both developing cerebellar and adult dosal root ganglion neurons. In contrast, MAG promotes neurite outgrowth from newborn DRG neurons. Thus, MAG may be responsible for the lack of CNS nerve regeneration and may influce both temporally and spatially regeneration in the PNS.

References

1. Quarles RH. 2007, J Neurochem. 100 (6): 1431-48.
2. Mukhopadhyay G. et al., 1994, Neuron. 13 (3): 757-67.
3. Barton DE. et al., 1987, Genomics. 1 (2): 107-12.