Anti-M13 Antibody

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Anti-M13 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-M13 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: other
Specificity
other M13
Immunogen
M13 Bacteriophage
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 Bacteriophage. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #MM05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-M13 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 0.1-0.4 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-M13 Antibody (Mouse Monoclonal antibody) Images

Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP

M13 Background Information

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single stranded genome it packages. The general stages to a viral life cycle are: infection, replication of the viral genome, assembly of new viral particles and then release of the progeny particles from the host. Filamentous phage use a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single stranded DNA genome to a double stranded replicative form.
References
  • Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22.
  • Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86.
  • Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63.
  • Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6.
  • Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.
  • Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Author
    Qiang, X;Sun, K;Xing, L;Xu, Y;Wang, H;Zhou, Z;Zhang, J;Zhang, F;Caliskan, B;Wang, M;Qiu, Z;
    Year
    2017
    Journal
    Sci Rep
    Application
    Phage ELISA assay
  • Identification of novel peptides against TNF-α using phage display technique and in silico modeling of their modes of binding
    Author
    Alizadeh, AA;Hamzeh-Mivehroud, M;Farajzadeh, M;Dastmalchi, S;
    Year
    2017
    Journal
    Eur J Pharm Sci
    Application
    ELISA
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