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Listeria monocytogenes flagellin / flaA Antibody, Rabbit MAb

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Listeria monocytogenes FLAA Antibody Product Information
Immunogen:Recombinant Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein (Catalog#40067-V07E)
Clone ID:121
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Listeria monocytogenes Listeria monocytogenes flagellin / flaA (Catalog#40067-V07E; YP_013330.1; Met1-Ser287).
Listeria monocytogenes FLAA Antibody Usage Guide
Specificity:Listeria monocytogenes Listeria monocytogenes flagellin / FlaA
Application:ELISA, IHC-P, ICC/IF, IF, FCM (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Listeria monocytogenes Listeria monocytogenes flagellin / FlaA. The detection limit for Listeria monocytogenes Listeria monocytogenes flagellin / FlaA is approximately 0.0195 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other FLAA Antibody Products
Listeria monocytogenes flagellin / FlaA Background

The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases.

Listeria monocytogenes Listeria monocytogenes flagellin / FlaA References
  • Gorski L. et al., 2009, PLoS ONE. 4 (4): E5142.
  • Tresse O. et al., 2009, Can J Microbiol. 55: 189-96.
  • Schirm M. et al., 2004, J Bacteriol. 186: 6721-7.
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