|IP||1-2 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-LTA4H rabbit monoclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lane B: A549 Whole Cell Lysate
Lane C: 293T Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:69 kDa
Observed band size:69 kDa
LTA4H was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate0.5 µL anti-LTA4H rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 110 kDa
Observed band size: 110 kDa
Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Leukotriene A4 Hydrolase Antibody||Rabbit||Polyclonal||ELISA||50268-RP01|
|Anti-Leukotriene A4 Hydrolase Antibody||Rabbit||Polyclonal||WB,ELISA,IP||50268-RP02|
|Anti-Leukotriene A4 Hydrolase Antibody||Rabbit||Monoclonal||ELISA||10276-R455|
|Anti-Leukotriene A4 Hydrolase Antibody||Rabbit||Polyclonal||WB,ELISA,IP||10276-RP02|
|Anti-Leukotriene A4 Hydrolase Antibody||Rabbit||Monoclonal||ELISA||10276-R457|