Leukotriene A4 Hydrolase / LTA4H Antibody

Leukotriene A4 Hydrolase / LTA4H Antibody Datasheet

Leukotriene A4 Hydrolase / LTA4H Antibody Product Information

• Immunogen: Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein (Catalog#10276-H08B)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 201
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Leukotriene A4 Hydrolase / LTA4H (rh Leukotriene A4 Hydrolase / LTA4H; Catalog#10276-H08B; NP_000886.1; Met1-Asp611, F578V).

Leukotriene A4 Hydrolase / LTA4H Antibody Usage Guide

• Specificity: Human Leukotriene A4 Hydrolase / LTA4H
• No cross-reactivity in ELISA with
  

  Mouse LTA4H

• Western blot: This antibody can be used at 5-10 μg/mL with the appropriate secondary reagents to detect Human Leukotriene A4 Hydrolase / LTA4H in WB.

  A B C Sample (whole cell lysate) Hela A549 293T Sample Volume (cells/lane) 2.5×105 5×105 5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 5-10 μg/ml Secondary Antibody Dylight 800-Labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LTA4H.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Leukotriene A4 Hydrolase / LTA4H Antibody Background

The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is thus regarded as a therapeutic target for inflammation.

References

1. Mancini, J.A. and Evans. J.F. 1995, Eur. J. Biochem. 231: 65-71.
2. Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-11273.
3. Rudberg, P.C. et al., 2004, J. Biol. Chem. 279: 27376-27382.
4. Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-8166.