Anti-Leukotriene A4 Hydrolase Antibody

Cat: 10276-R201
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Anti-Leukotriene A4 Hydrolase Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Leukotriene A4 Hydrolase Antibody
Validated applications
WB,ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Leukotriene A4 Hydrolase
No cross activity in ELISA with Mouse LTA4H
Immunogen
Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein (Catalog#10276-H08B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Leukotriene A4 Hydrolase / LTA4H (rh Leukotriene A4 Hydrolase / LTA4H; Catalog#10276-H08B; NP_000886.1; Met 1-Asp 611).
Source
Monoclonal Rabbit IgG Clone #201
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Leukotriene A4 Hydrolase Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
ICC-IF 1:100-1:500  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Leukotriene A4 Hydrolase Antibody (Rabbit Monoclonal antibody) Images
Human Leukotriene A4 Hydrolase/LTA4H Immunohistochemistry(IHC) 2893
Immunofluorescence staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human Leukotriene A4 Hydrolase/LTA4H Western blot (WB) 13159

Anti-LTA4H rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:69 kDa

Observed band size:69 kDa

Human Leukotriene A4 Hydrolase/LTA4H Immunoprecipitation(IP) 17667

LTA4H was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-LTA4H rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 110 kDa

Observed band size: 110 kDa

Anti-Leukotriene A4 Hydrolase Antibody Alternative Names
Anti-LAT4 Antibody
Leukotriene A4 Hydrolase Background Information

Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

Full Name
leukotriene A4 hydrolase
References
  • Mancini, JA. et al.,1995, Eur. J. Biochem. 231: 65-71.
  • Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73.
  • Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82.
  • Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Leukotriene A4 Hydrolase Antibody Rabbit Polyclonal WB,ELISA,IP 50268-RP02
    Anti-Leukotriene A4 Hydrolase Antibody Rabbit Polyclonal ELISA 50268-RP01
    Anti-Leukotriene A4 Hydrolase Antibody Rabbit Polyclonal WB,ELISA,IP 10276-RP02
    Anti-Leukotriene A4 Hydrolase Antibody Rabbit Monoclonal ELISA 10276-R455
    Anti-Leukotriene A4 Hydrolase Antibody Rabbit Monoclonal ELISA 10276-R457
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