Human Leptin Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10221-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000230.2

cDNA Size:    504

Synonym:   LEP, OB, OBS, FLJ94114

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens leptin DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA
61       GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
121      AGGATCAATG ACATTTCACA CACGCAGTCA GTCTCCTCCA AACAGAAAGT CACCGGTTTG
181      GACTTCATTC CTGGGCTCCA CCCCATCCTG ACCTTATCCA AGATGGACCA GACACTGGCA
241      GTCTACCAAC AGATCCTCAC CAGTATGCCT TCCAGAAACG TGATCCAAAT ATCCAACGAC
301      CTGGAGAACC TCCGGGATCT TCTTCACGTG CTGGCCTTCT CTAAGAGCTG CCACTTGCCC
361      TGGGCCAGTG GCCTGGAGAC CTTGGACAGC CTGGGGGGTG TCCTGGAAGC TTCAGGCTAC
421      TCCACAGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGTCTC TGCAGGACAT GCTGTGGCAG
481      CTGGACCTCA GCCCTGGGTG CTGA

Quality Control: cDNA sequencing results is available upon request.

Leptin related areas, pathways, and other information

Cytokine