Leptin Receptor / CD295 / LEPR Antibody (Cytokine)

Leptin Receptor / CD295 / LEPR Antibody Datasheet

Leptin Receptor / CD295 / LEPR Antibody Product Information

• Immunogen: Recombinant Human Leptin Receptor / CD295 / LEPR protein (Catalog#10322-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7C3F4
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Leptin Receptor / CD295 / LEPR (rh Leptin Receptor / CD295 / LEPR; Catalog#10322-H08H; NP_002294.2; Met 1-Asp 839). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Leptin Receptor / CD295 / LEPR Antibody Usage Guide

• Specificity: Human Leptin Receptor / CD295 / LEPR
• No cross-reactivity in ELISA with

  Human LEP

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human LEPR in WB. Using a DAB detection system, the detection limit for Human LEPR is approximately 4 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human LEPR. The detection limit for Human LEPR is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Leptin Receptor / CD295 / LEPR Antibody Background

Leptin receptor (LEPR), also known as OB-R and B219, is a single-transmembrane-domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.

References

1. Tartaglia, L.A. et al., 1995, Cell. 83: 1263-1271.
2. Cioffi, J.A. et al., 1996, Nature Medicine 2: 585-589.
3. Couturier, C. et al., 2003, J. Biol. Chem. 278: 26604-26611.