Legumain Protein (LGMN Protein)

Legumain Products

Legumain Protein, Recombinant

Molecule	Species	Description	Cat. No
Legumain/LGMN	Mouse	Legumain/LGMN Protein, Recombinant	50051-M07H

Legumain cDNA Clone

Molecule	Species	Description	Cat. No
Legumain/LGMN	Mouse	Mus musculus Legumain/LGMN cDNA Clone	MG50051-M

Legumain Related Areas

Enzyme>>Protease & Regulator>>Cysteine Protease & Regulator>>Legumain/LGMN

Immunology>>Innate Immunity>>Lysosomal Enzyme>>Legumain/LGMN

Legumain Alternative Names

Legumain, LGMN, AEP, PRSC1, LGMN1 [Homo sapiens]

Legumain, Lgmn, AEP, Prsc1, AI746452, AU022324 [Mus musculus]

Legumain Background

The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.

Legumain Related Studies

1. Schwarz G, et al. (2002) Characterization of legumain. Biol Chem. 383(11): 1813-6.
2. Liu C, et al. (2003) Overexpression of legumain in tumors is significant for invasion/metastasis and a candidate enzymatic target for prodrug therapy. Cancer Res. 63(11): 2957-64.
3. Murthy RV, et al. (2005) Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer. Clin Cancer Res. 11(6): 2293-9.
4. Gawenda J, et al. (2007) Legumain expression as a prognostic factor in breast cancer patients. Breast Cancer Res Treat. 102(1): 1-6.
5. Clerin V, et al. (2007) Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 201(1): 53-66.
6. Lewēn S, et al. (2008) A Legumain-based minigene vaccine targets the tumor stroma and suppresses breast cancer growth and angiogenesis. Cancer Immunol Immunother. 57(4): 507-15.