Layilin Antibody (Antigen Affinity Purified)

Layilin / LAYN Antibody (Antigen Affinity Purified) Datasheet

Layilin / LAYN Antibody Product Information

• Immunogen: Recombinant Human Layilin / LAYN protein (Catalog#10208-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human Layilin / LAYN (rh Layilin / LAYN; Catalog#10208-H08H; NP_849156.1; Met 1- Glu 220). Layilin / LAYN specific IgG was purified by Human Layilin / LAYN affinity chromatography.

Layilin / LAYN Antibody Usage Guide

• Specificity: Human Layilin / LAYN
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human layilin in WB. Using a DAB detection system, the detection limit for Human Layilin is approximately 0.5 ng/lane under reducing conditions and non-reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human layilin. The detection limit for Human layilin is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Layilin / LAYN Antibody Background

Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction.

References

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5. Schoenwaelder, S.M. et al., 1999, Curr Opin Cell Biol. 11:274–286.