Anti-LAYN Antibody

Cat: 10208-R107
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Anti-LAYN Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-LAYN Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human LAYN
Immunogen
Recombinant Human Layilin protein (Catalog#10208-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Layilin (rh Layilin; Catalog#10208-H08H; NP_849156.1; Met 1-Glu 220).
Source
Monoclonal Rabbit IgG Clone #107
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-LAYN Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LAYN Antibody Alternative Names
Anti-Layilin Antibody
LAYN Background Information

Layilin, a recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, a ten-amino acid motif in the layilin cytoplasmic domain is sufficient for talin binding, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Furthermore, talin binds layilin, PIPK1gamma and integrins in similar although subtly different ways. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that layilin may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. In addition, layilin might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.

Full Name
layilin
References
  • Borowsky ML, et al. (1998) Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles.J Cell Biol. 143(2):429-42.
  • Bono P, et al. (2001) Layilin, a novel integral membrane protein, is a hyaluronan receptor. Mol Biol Cell. 12(4)891-900.
  • Bono P, et al. (2005) Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin. Exp Cell Res. 308(1):177-87.
  • Scoles DR. (2007) The merlin interacting proteins reveal multiple targets for NF2 therapy. Biochim Biophys Acta. 1785(1):32-54.
  • Chen Z, et al. (2008) Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells. Biotechnol Appl Biochem. 50(Pt 2):89-96.
  • Wegener KL, et al. (2008) Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain. J Mol Biol. 382(1):112-26.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LAYN Antibody Rabbit Polyclonal ELISA 90160-T16
    Anti-LAYN Antibody Rabbit Polyclonal ELISA,IHC-P 80431-T24
    Anti-LAYN Antibody Rabbit Polyclonal ELISA 80431-RP01
    Anti-LAYN Antibody Mouse Monoclonal ELISA 10208-MM02
    Anti-LAYN Antibody Mouse Monoclonal ELISA,ELISA(Cap) 10208-MM07
    Anti-LAYN Antibody Rabbit Polyclonal ELISA 10208-RP01
    Anti-LAYN Antibody Mouse Monoclonal ELISA,ELISA(Det) 10208-MM08
    Anti-LAYN Antibody Rabbit Polyclonal ELISA 10208-RP02
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