|IP||4-6 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
LXN was immunoprecipitated using:
Lane A:0.5 mg A549 Whole Cell Lysate
Lane B:0.5 mg Hela Whole Cell Lysate2 µL anti-LXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-LXN rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 25.7 kDa
Observed band size: 25.7 kDa
Anti-LXN rabbit monoclonal antibody at 1:500 dilution
Lane A: A549 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:26 kDa
Observed band size:26 kDa
Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Latexin Antibody (FITC)||Rabbit||Monoclonal||FCM||10211-R101-F|
|Anti-Latexin Antibody (FITC)||Mouse||Monoclonal||FCM||10211-MM02-F|
|Anti-Latexin Antibody (PE)||Rabbit||Monoclonal||FCM||10211-R101-P|
|Anti-Latexin Antibody (PE)||Mouse||Monoclonal||FCM||10211-MM02-P|