Anti-Latexin Antibody

Cat: 10211-R101
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Anti-Latexin Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Latexin Antibody
Validated applications
WB,ELISA,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Latexin
Immunogen
Recombinant Human Latexin / LXN Protein (Catalog#10211-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Latexin / LXN (rh Latexin / LXN; Catalog#10211-H07E; NP_064554.3; Glu 2-Glu 222) .
Source
Monoclonal Rabbit IgG Clone #101
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Latexin Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Latexin Antibody (Rabbit Monoclonal antibody) Images
Human Latexin Immunofluorescence(IF) 2889
Immunofluorescence staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human Latexin Flow Cytometry (FC) 5098
Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human TFPI  Immunoprecipitation(IP) 8002

LXN was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-LXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LXN rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25.7 kDa

Observed band size: 25.7 kDa

Human Latexin Western blot (WB) 13145

Anti-LXN rabbit monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:26 kDa

Anti-Latexin Antibody Alternative Names
Anti-ECI Antibody;Anti-TCI Antibody
Latexin Background Information

Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.

Full Name
latexin
References
  • Liu Q., et al.,(2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.
  • Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.
  • Muzny D.M., et al.,(2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Latexin Antibody Rabbit Monoclonal ELISA 50050-R009
    Anti-Latexin Antibody Rabbit Monoclonal ELISA 50050-R040
    Anti-Latexin Antibody Rabbit Polyclonal WB,ELISA,IP 50050-RP02
    Anti-Latexin Antibody Rabbit Polyclonal ELISA 50050-RP01
    Anti-Latexin Antibody (FITC) Rabbit Monoclonal FCM 10211-R101-F
    Anti-Latexin Antibody Rabbit Polyclonal WB,ELISA 10211-RP01
    Anti-Latexin Antibody Rabbit Polyclonal WB,ELISA,IP 10211-RP02
    Anti-Latexin Antibody Mouse Monoclonal FCM,ICC/IF,IF 10211-MM02
    Anti-Latexin Antibody (FITC) Mouse Monoclonal FCM 10211-MM02-F
    Anti-Latexin Antibody (PE) Rabbit Monoclonal FCM 10211-R101-P
    Anti-Latexin Antibody (PE) Mouse Monoclonal FCM 10211-MM02-P
    Anti-Latexin Antibody Mouse Monoclonal WB,ELISA,IP 10211-MM04
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