After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Latexin / LXN Antibody, Rabbit MAb

DatasheetReviewsRelated ProductsProtocols
Human Latexin Antibody Product Information
Immunogen:Recombinant Human Latexin / LXN Protein (Catalog#10211-H07E)
Clone ID:101
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human Latexin / LXN (rh Latexin / LXN; Catalog#10211-H07E; NP_064554.3; Glu 2-Glu 222) .
Human Latexin Antibody Usage Guide
Specificity:Human Latexin / LXN
Application:WB, ELISA, ICC/IF, IF, IP, FCM

WB: 0.5-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LXN. The detection limit for Human LXN is approximately 0.00245 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

IP: 4-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human Latexin Antibody WB Application Image
Latexin / LXN Antibody, Rabbit MAb, Western blot
Human Latexin Antibody IF Application Image
[Click to enlarge image]
Immunofluorescence staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Latexin / LXN Antibody, Rabbit MAb, Immunofluorescence
Human Latexin Antibody FC Application Image
[Click to enlarge image]
Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Latexin / LXN Antibody, Rabbit MAb, Flow cytometric analysis
Human Latexin Antibody IP Application Image
Latexin / LXN Antibody, Rabbit MAb, Immunoprecipitation
Other Latexin Antibody Products
Latexin Background

Mouse Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.

Product nameProduct name
Size / Price
Catalog: 10211-R101-50
List Price:   (Save )
Price:      [How to order]
AvailabilityIn Stock
 Shipping instructions
All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.