Mouse LYVE1 / LYVE-1 Protein

Lymphatic vessel endothelial hyaluronan receptor 1 Protein Datasheet

LYVE1 / LYVE-1 Protein Product Information

• Synonym: LYVE1, Crsbp1, Xlkd1
• Protein Construction: A DNA sequence encoding the mouse LYVE1 (Q8BHC0) (Met1- Gly228 ) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

LYVE1 / LYVE-1 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 24
• Molecular Mass: The recombinant mouse LYVE1 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 446 amino acids and has a predicted molecular mass of 49.1 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LYVE1 / LYVE-1 protein

LYVE1 / LYVE-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LYVE1 / LYVE-1 Protein Description

YVE1, also known as LYVE-1, is a type I integral membrane glycoprotein and acts as a receptor and binds to both soluble and immobilized hyaluronan. It contains 1 Link domain and mainly expressed in endothelial cells lining lymphatic vessels. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis. It may act as an hyaluronan transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. LYVE1 also is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, and sort these cells for experimental purposes. It also functions as a ligand-specific transporter trafficking between intracellular organelles and the plasma membrane. It plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding.

References

1. Jackson DG. 2003, Trends Cardiovasc Med. 13 (1): 1-7.
2. Banerji S. et al., 1999, J Cell Biol. 144 (4): 789-801.
3. Mouta Carreira C. et al., 2001, Cancer Res. 61 (22): 8079-84.
4. Cursiefen C. et al., 2002, Invest Ophthalmol Vis Sci. 43 (7): 2127-35.