LY9 / CD229 / SLAMF3 Antibody

LY9 / CD229 / SLAMF3 Antibody Datasheet

LY9 / CD229 / SLAMF3 Antibody Product Information

• Immunogen: Recombinant Mouse LY9 / CD229 / SLAMF3 protein (Catalog#50504-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 107
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LY9 / CD229 / SLAMF3 (rM LY9 / CD229 / SLAMF3; Catalog#50504-M08H; NP_032560.2; Met 1-Arg 453).

LY9 / CD229 / SLAMF3 Antibody Usage Guide

• Specificity: Mouse LY9 / CD229 / SLAMF3
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse LY9. The detection limit for Mouse LY9 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LY9 / CD229 / SLAMF3 Antibody Background

T-lymphocyte surface antigen Ly-9, also known as Cell surface molecule Ly-9, Lymphocyte antigen 9, CD229 and LY9, is a single-pass type I membrane protein. Mouse CD229 / LY9 is a cell surface molecule of the CD150 (signaling lymphocyte activation molecule) family. CD229 / LY9 contains oneIg-like C2-type (immunoglobulin-like) domains and oneIg-like V-type (immunoglobulin-like) domains. This family consists of nine leukocyte receptors of the immunoglobulin superfamily that are involved in leukocyte activation. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. The cytoplasmic tail of CD229 binds to SAP, a protein that is defective in X-linked lymphoproliferative syndrome. CD229 is its own ligand and participates in the immunological synapse. CD229 cell surface expression on lymphocytes surface is strongly and differentially regulated within the CD150 family members. CD229 is a pan-lymphocyte marker and mAbs against CD229 are able to down-modulate T-cell activation. CD229 / LY9 may participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction.

References

1. Del Valle,J.M. et al., 2003, J Biol Chem.278 (19): 17430-7.
2. Romero,X. et al., 2005,J Immunol. 174 (11): 7033-42.
3. Martin,M. et al., 2005, J Immunol. 174 (10): 5977-86.
4. Bund,D. et al., 2006,Exp Hematol. 34 (7): 860-9.
5. Sintes,J. et al., 2007, Tissue Antigens. 70 (5): 355-62.