LTC4S / LTC4 synthase Protein (His Tag)

Leukotriene C4 Synthase / LTC4 synthase Protein

LTC4 synthase / LTC4S Protein Product Information

• Synonym: MGC33147, LTC4S
• Protein Construction: A DNA sequence encoding the human LTC4S (NP_665874.1) (Met 1- Ala 150) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

LTC4 synthase / LTC4S Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human LTC4S consists of 156 amino acids and predicts a molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Hepes, 0.1% Triton 0.5% DOC, 10% Gly, pH 8.0
• SDS-PAGE: LTC4S protein

LTC4 synthase / LTC4S Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LTC4 synthase / LTC4S Protein Description

Leukotriene C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S, is a multi-pass membrane protein which belongs to the MAPEG family. LTC4S is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelets. LTC4S is essential for the production of cysteinyl leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the conjugation function of human LTC4S has identified R51 and Y93 as critical for acid and base catalysis of LTA4 and reduced glutathione, respectively. A comparison across species for proteins that possess LTC4S activity reveals conservation of both of these residues, whereas R51 is absent in the FLAP molecules. Thus, within the glutathione S-transferase superfamily of genes, alignment of specific residues allows the separation of LTC4S family members from their most structurally similar counterparts, the FLAP molecules. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.

References

1. Gupta, N. et al., 1999, FEBS Lett. 449 (1): 66-70.
2. Penrose, JF. et al., 1999, Allergy Asthma Proc. 20 (6): 353-60.
3. Sayers, I. et al., 2003, Thorax. 58 (5): 417-24.
4. Schröder, O. et al., 2005, Cell Mol Life Sci. 62 (1): 87-94.