Cynomolgus LTBR Protein (Cytokine)

Lymphotoxin beta receptor (TNFR superfamily, member 3) Protein Datasheet

LTBR Protein Product Information

• Synonym: LTBR
• Protein Construction: A DNA sequence encoding the cynomolgus LTBR(F6V995) (Met1-Met225) was expressed with the Fc region of human IgG1 at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

LTBR Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 28
• Molecular Mass: The recombinant cynomolgus LTBR is a disulfide-linked homodimer. The reduced monomer comprises 439 amino acids and has a calculated molecular mass of 48.8 KDa. The apparent molecular mass of the protein is approximately 64-70 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LTBR protein

LTBR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LTBR Protein Description

LTBR (lymphotoxin beta receptor (TNFR superfamily, member 3)) contains 4 TNFR-Cys repeats and belongs to the tumor necrosis factor (TNF) family of receptors. It is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. It is the receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. It promotes apoptosis via TRAF3 and TRAF5 and may play a role in the development of lymphoid organs. LTBR has been shown to interact with diablo homolog and TRAF3. It can lead to the release of the cytokine interleukin 8. Overexpression of LTBR in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release. LTBR is also essential for development and organization of the secondary lymphoid organs and chemokine release. It specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and transformed cells.

References

1. Summers deLuca L. et al., 2011, Proc Natl Acad Sci. 108 (5): 2046-51.
2. Bista P. et al., 2010, J Biol Chem. 285 (17): 12971-8.
3. Xu Y. et al., 2011, Biochem Biophys Res Commun. 404 (4): 1060-4.