Cynomolgus monkey LTB Gene cDNA Clone / ORF Clone

LTB cDNA Clone Datasheet

LTB cDNA Clone Product Information

• cDNA Size: 735
• Gene Synonym: LTB
• Source: Cynomolgus monkey

LTB cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) lymphotoxin beta (TNF superfamily, member 3) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 369 T/A resulting in the amino acid Asp substitution by Asn and 198 A/G not causing the amino acid variation.
  Please check the sequence information before order.

LTB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LTB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LTB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGCAC TGGGGCTGGA GGGCAGGGGT GGGAGGCTCC AGGGGAGGGG CTCCCTCCTG
61       CTAGCTGTGG CAGGAGCCAC TTCTCTGGTG ACCCTGTTGC TGGCGGTGCC TATCACTGTC
121      CTGGCTGTGC TGGCCTTAGT GCCCCAGGAT CAGGGAGGAC TGGTAACGGA CACCGCTGAC
181      CCCGGGGCAC AGGCCCAGCA AGGACTGGGG TTTCAGAAGC TGCCGGAGGA GGAGCCAGAA
241      GCAGATCTCA GCCCCGGGCT CCCAGCTGCC CACCTCATAG GCGCTCCGCT GAAGGGGCAG
301      GGGCTAGGCT GGGAGGCGAC GAAGGAACAG GCGTTTCTGA CGAGCGGGAC GCAGTTCTCG
361      GACGCCGAAG GGCTGGCGCT CCCGCAGGAC GGCCTCTATT ACCTCTACTG TCTCGTCGGC
421      TACCGGGGCC GGGCGCCCCC TGGCGGCGCG GAACCCCGGG GCCGCTCGGT CACGCTGCGC
481      AGCTCTCTGT ACCGGGCGGG GGGCGCCTAC GGGCCGGGCA CTCCGGAGCT GCTGCTCGAG
541      GGCGCTGAGA CCGTGACTCC CGTGCTGGAC CCGGCCGGGA GGCAAGGGTA CGGGCCTCTC
601      TGGTACACAA GCGTGGGGTT CGGCGGCCTG GTGCAGCTCC GGAGGGGCGA GAGGGTGTAC
661      GTCAACATCA GTCACCCCGA TATGGTGGAC TTCGCGAGAG GGAAGACCTT CTTTGGGGCC
721      GTGATGGTGG GGTGA

 

Quality Control:cDNA sequencing results is available upon request.