The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rhesus LTA ORF mammalian expression plasmid, C-GFPSpark tag||CG90086-ACG|
|Rhesus LTA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90086-ACR|
|Rhesus LTA ORF mammalian expression plasmid, C-Flag tag||CG90086-CF|
|Rhesus LTA ORF mammalian expression plasmid, C-His tag||CG90086-CH|
|Rhesus LTA ORF mammalian expression plasmid, C-Myc tag||CG90086-CM|
|Rhesus LTA ORF mammalian expression plasmid, C-HA tag||CG90086-CY|
|Rhesus LTA ORF mammalian expression plasmid, N-Flag tag||CG90086-NF|
|Rhesus LTA ORF mammalian expression plasmid, N-His tag||CG90086-NH|
|Rhesus LTA ORF mammalian expression plasmid, N-Myc tag||CG90086-NM|
|Rhesus LTA ORF mammalian expression plasmid, N-HA tag||CG90086-NY|
|Rhesus LTA natural ORF mammalian expression plasmid||CG90086-UT|
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Lymphotoxin-alpha, also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA TNFSF1 and TNFB, is a secreted protein which belongs to the tumor necrosis factor family. TNF-beta/TNFSF1/Lymphotoxin alpha is a highly inducible, secreted, and exists as homotrimeric molecule. It is a cytokine that in its homotrimeric form binds to TNFRSF1A / TNFR1, TNFRSF1B / TNFBR and TNFRSF14 / HVEM. In its heterotrimeric form with LTB, TNF-beta/TNFSF1/Lymphotoxin alpha binds to TNFRSF3 / LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells. TNF-beta/TNFSF1/Lymphotoxin alpha forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. TNF-beta/TNFSF1/Lymphotoxin alpha is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in TNF-beta/TNFSF1/Lymphotoxin alpha are a cause of susceptibility psoriatic arthritis which is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy.