Anti-LRPAP1 Antibody

Cat: 50281-R009
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Anti-LRPAP1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-LRPAP1 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse LRPAP1
No cross-reactivity in ELISA with Human LRPAP1
Immunogen
Recombinant Mouse LRPAP1 protein (Catalog#50281-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LRPAP1 (rM LRPAP1; Catalog#50281-M08H; NP_038615.2; Gln 29-Leu 360).
Source
Monoclonal Rabbit IgG Clone #009
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-LRPAP1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:500-1:2000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LRPAP1 Antibody (Rabbit Monoclonal antibody) Images
Mouse LRPAP1/A2MRAP Immunohistochemistry(IHC) 3244
Immunochemical staining of mouse LRPAP1 in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.
Mouse LRPAP1/A2MRAP Immunohistochemistry(IHC) 3246
Immunochemical staining of mouse LRPAP1 in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nephric tubule.
Human MESDC2 Immunoprecipitation(IP) 8033

Mouse LRPAP1 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

0.5 µL anti-Mouse LRPAP1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse LRPAP1 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 41 kDa

Observed band size: 41 kDa

Mouse LRPAP1/A2MRAP Western blot (WB) 17506

Anti-LRPAP1 rabbit monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:41 kDa

Observed band size:41 kDa

Anti-LRPAP1 Antibody Alternative Names
Anti-AA617339 Antibody;Anti-AI790446 Antibody;Anti-AU042172 Antibody;Anti-C77774 Antibody;Anti-HBP44 Antibody;Anti-RAP Antibody
LRPAP1 Background Information

Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiosis. The genetic variations at the LRPAP1 locus may modulate Alzheimer disease (AD) phenotype beyond risk for disease. In addition, the variation at the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

Full Name
low density lipoprotein receptor-related protein associated protein 1
References
  • Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.
  • Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19?
  • Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.
  • Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LRPAP1 Antibody Rabbit Monoclonal IHC-P 50281-R011
    Anti-LRPAP1 Antibody Rabbit Polyclonal ELISA 50281-RP01
    Anti-LRPAP1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50281-RP02
    Anti-LRPAP1 Antibody Mouse Monoclonal WB 11100-MM34
    Anti-LRPAP1 Antibody Rabbit Polyclonal IHC-P,ICC/IF,IF 107474-T10
    Anti-LRPAP1 Antibody Mouse Monoclonal ELISA 11100-M105
    Anti-LRPAP1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 11100-RP02
    Anti-LRPAP1 Antibody Mouse Monoclonal WB,ELISA,IHC-P,IP 11100-MM01
    Anti-LRPAP1 Antibody Mouse Monoclonal IHC-P 11100-M003
    Anti-LRPAP1 Antibody Mouse Monoclonal WB 11100-M034
    Anti-LRPAP1 Antibody Rabbit Monoclonal WB,ELISA,ICC/IF,IF,IP 11100-R004
    Anti-LRPAP1 Antibody Rabbit Polyclonal ELISA 11100-RP01
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