LRPAP1 / A2MRAP Antibody

LRPAP1 / A2MRAP Antibody

LRPAP1 / A2MRAP Antibody Product Information

• Immunogen: Recombinant human LRPAP1 protein ( Catalog#11100-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 4
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human LRPAP1 / A2MRAP ( rh LRPAP1 ; Catalog#11100-H08H ; Tyr 35 - Leu 357 ; NP_002328.1 ).

LRPAP1 / A2MRAP Antibody Usage Guide

• Specificity: Human LRPAP1 / A2MRAP
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human LRPAP1 in WB. Using a DAB detection system, the detection limit for human LRPAP1 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1- 0.2 μg/mL with the appropriate secondary reagents to detect human LRPAP1. The detection limit for LRPAP1 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LRPAP1 / A2MRAP Antibody Background

Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin.

References

1. Liu, CX. et al., 2001, J. Biol. Chem. 276: 28889-96.
2. Schutte DL, et al., 2003, Res Theory Nurs Pract .17 (4): 301-19.
3. Jensen, GA. et al., 2006, J. Mol. Biol. 362: 700-16.
4. Santovito, A. et al., 2007, Hum Biol. 79 (6): 699-706.