LRPAP1 Antibody

LRPAP1 Antibody Datasheet

LRPAP1 Antibody Product Information

• Immunogen: Recombinant Human LRPAP1 protein (Catalog#11100-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 34
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LRPAP1 (rh LRPAP1; Catalog#11100-H08H; NP_002328.1; Tyr 35-Leu 357). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

LRPAP1 Antibody Usage Guide

• Specificity: Human LRPAP1
• Western blot: This antibody can be used at 2-5 μg/mL with the appropriate secondary reagents to detect Human LRPAP1 in WB.

  A B Sample (whole cell lysate) 293T A549 Sample Volume (cells/lane) 5×105 5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 2-5 μg/ml Secondary Antibody Dylight 800-labeled Antibody To Mouse IgG (H+L), at 1:20000 dilution.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LRPAP1 Antibody Background

Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of thethyroid, where it can serve as a receptor for the proteinthyroglobulin.

References

1. Liu, CX. et al., 2001, J. Biol. Chem. 276: 28889-96.
2. Schutte DL, et al., 2003,Res Theory Nurs Pract.17 (4): 301-19.
3. Jensen, GA. et al., 2006, J. Mol. Biol. 362: 700-16.
4. Santovito, A. et al., 2007, Hum Biol. 79 (6): 699-706.