LRP10 Protein

Low density lipoprotein receptor-related protein 10 Protein Datasheet

LRP10 Protein Product Information

• Synonym: MSTP087, LRP9, MST087
• Protein Construction: A DNA sequence encoding the human LRP10 (Q7Z4F1-1) extracellular domain (Met 1- Lys 440) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

LRP10 Protein QC Testing

• Purity: >88 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His 17
• Molecular Mass: The secreted recombinant human LRP10/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 665 amino acids and has a predicted molecular mass of 73 kDa. The apparent molecular mass of rhLRP10/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LRP10 protein

LRP10 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LRP10 Protein Description

Low-density lipoprotein receptor-related protein 10 (LRP10) is one member of the Low-density lipoprotein receptor-related protein family. LRP10 plays an important role in lipoprotein metabolism. Chylomicron remnants, produced by lipolysis, are rapidly taken up by the liver via an apolipoprotein E (apoE)-mediated, receptor-dependent process. The low density lipoprotein (LDL) receptor-related protein (LRP) has been suggested as the potential apoE receptor. low density lipoprotein (LDL) receptor-related protein (LRP) 10 (LRP10) inhibits the canonical Wnt / beta- catenin signaling pathway. The beta-catenin/T cell factor (TCF) transcriptional activity in HEK293 cells was activated by transfection with Wnt3a or LRP6, which was then inhibited by co-transfection with LRP10. Deletion of the extracellular domain of LRP10 negated its inhibitory effect. It sugested that LRP10 may interfere with the formation of the beta-catenin/TCF complex and its binding to target DNA in the nucleus, and that the extracellular domain of LRP10 is critical for inhibition of the canonical Wnt/beta-catenin signaling pathway. LRP10 has been improved to play a significant role in the brain physiology other than lipoprotein metabolism.

References

1. Jeong YH. et al., 2010, Biochem Biophys Res Commun. 392 (4): 495-9.
2. Beisiegel U. et al., 1991, Proc Natl Acad Sci. 88 (19): 8342-6.
3. Strickland DK. et al., 1990, J Biol Chem. 265 (29): 17401-4.