Human LRAT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13463-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LRAT cDNA Clone Datasheet

LRAT cDNA Clone Product Information

Gene Bank Ref.ID:	BC031053
cDNA Size:	693
Gene Synonym:	LCA14; LRAT
Source:	Human

LRAT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

LRAT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LRAT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LRAT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC
61       AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT
121      GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC
181      TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC
241      CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC
301      GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT
361      AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG
421      GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC
481      TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG
541      TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC
601      TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT
661      TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

Quality Control:cDNA sequencing results is available upon request.