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Rat beta globin minor gene Gene ORF cDNA clone in cloning vector

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Rat LOC100134871 cDNA Clone Product Information
NCBI RefSeq:NM_001113223.1
RefSeq ORF Size:444bp
cDNA Description:Full length Clone DNA of Rattus norvegicus beta globin minor gene.
Gene Synonym:LOC100134871
Species:Rat
Vector:pUC19 Vector
Plasmid:cpUC19-ratLOC100134871
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 60T>C, 165C>T, 375C>G, 405G>A and 426G>T not causing the amino acid variation; 61G>C (A21P) and 377G>C (C126S).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Rat LOC100134871 Gene Plasmid Map
Rat LOC100134871 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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  • Rat LOC100134871 Gene cDNA Clone (full-length ORF Clone)
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