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Rat LMAN2 Gene ORF cDNA clone in cloning vector

  • Rat LMAN2 Gene cDNA Clone (full-length ORF Clone)
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Rat LMAN2 cDNA Clone Product Information
NCBI RefSeq:NM_001115024.2
RefSeq ORF Size:1077bp
cDNA Description:Full length Clone DNA of Rattus norvegicus lectin, mannose-binding 2.
Gene Synonym:Vip36
Species:Rat
Vector:pUC19 Vector
Plasmid:cpUC19-ratLman2
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 812T>G(I271S).
Sequencing primers:M13-47 and RV-M
( We provide with LMAN2 qPCR primers for gene expression analysis, RP300986 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Rat LMAN2 Gene Plasmid Map
Rat LMAN2 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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References
  • Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21.
  • Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63.
  • Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9.
  • Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 ( Pt 17): 2813-21.
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    Catalog: RG81022-U
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