|Recombinant Human LMAN2 / VIP36 protein (Catalog#12455-H08E)|
|0.2 μm filtered solution in PBS|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; Catalog#12455-H08E; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
No cross-reactivity in ELISA with
E.coli cell lysate
WB: 5-10 μg/ml
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human LMAN2. The detection limit for Human LMAN2 is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Human LMAN2 in ELISA pair set (Catalog: # SEK12455). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12455-MM04).
IP: 4-6 μg/mg of lysate
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.