Anti-LMAN2 Antibody

Cat: 12455-MM02
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Anti-LMAN2 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-LMAN2 Antibody
Validated applications
WB,ELISA,ELISA(Det),IP
Species reactivity
Reacts with: Human
Specificity
Human LMAN2
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Human LMAN2 / VIP36 protein (Catalog#12455-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; Catalog#12455-H08E; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #02
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-LMAN2 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 4-6 μL/mg of lysate  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human LMAN2 in ELISA pair set (Catalog: # SEK12455). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12455-MM04).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LMAN2 Antibody (Mouse Monoclonal antibody) Images
Human LMAN2 Western blot (WB) 13492

Anti-LMAN2 mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:40 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

Human LMAN2 Immunoprecipitation(IP) 17335

LMAN2-His was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-LMAN2-His mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LMAN2-His mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Anti-LMAN2 Antibody Alternative Names
Anti-C5orf8 Antibody;Anti-GP36B Antibody;Anti-VIP36 Antibody
LMAN2 Background Information
Full Name
lectin, mannose binding 2
References
  • Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21.
  • Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63.
  • Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9.
  • Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 ( Pt 17): 2813-21.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LMAN2 Antibody Mouse Monoclonal ELISA(Cap) 12455-MM04
    info info
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