|IP||4-6 μL/mg of lysate|
|ELISA(Det)||1:1000-1:10000||This antibody will detect Human LMAN2 in ELISA pair set (Catalog: # SEK12455). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12455-MM04).|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-LMAN2 mouse monoclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: Hela Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:40 kDa
Observed band size:40 kDa
(We are unsure as to the identity of these extra bands.)
LMAN2-His was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate2 µL anti-LMAN2-His mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-LMAN2-His mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 40 kDa
Observed band size: 40 kDa