Human LKB1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10670-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000455.4

cDNA Size:   1302

Synonym:  PJS; LKB1; STK11

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens serine/threonine kinase 11 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGAGGTGG TGGACCCGCA GCAGCTGGGC ATGTTCACGG AGGGCGAGCT GATGTCGGTG
61       GGTATGGACA CGTTCATCCA CCGCATCGAC TCCACCGAGG TCATCTACCA GCCGCGCCGC
121      AAGCGGGCCA AGCTCATCGG CAAGTACCTG ATGGGGGACC TGCTGGGGGA AGGCTCTTAC
181      GGCAAGGTGA AGGAGGTGCT GGACTCGGAG ACGCTGTGCA GGAGGGCCGT CAAGATCCTC
241      AAGAAGAAGA AGTTGCGAAG GATCCCCAAC GGGGAGGCCA ACGTGAAGAA GGAAATTCAA
301      CTACTGAGGA GGTTACGGCA CAAAAATGTC ATCCAGCTGG TGGATGTGTT ATACAACGAA
361      GAGAAGCAGA AAATGTATAT GGTGATGGAG TACTGCGTGT GTGGCATGCA GGAAATGCTG
421      GACAGCGTGC CGGAGAAGCG TTTCCCAGTG TGCCAGGCCC ACGGGTACTT CTGTCAGCTG
481      ATTGACGGCC TGGAGTACCT GCATAGCCAG GGCATTGTGC ACAAGGACAT CAAGCCGGGG
541      AACCTGCTGC TCACCACCGG TGGCACCCTC AAAATCTCCG ACCTGGGCGT GGCCGAGGCA
601      CTGCACCCGT TCGCGGCGGA CGACACCTGC CGGACCAGCC AGGGCTCCCC GGCTTTCCAG
661      CCGCCCGAGA TTGCCAACGG CCTGGACACC TTCTCCGGCT TCAAGGTGGA CATCTGGTCG
721      GCTGGGGTCA CCCTCTACAA CATCACCACG GGTCTGTACC CCTTCGAAGG GGACAACATC
781      TACAAGTTGT TTGAGAACAT CGGGAAGGGG AGCTACGCCA TCCCGGGCGA CTGTGGCCCC
841      CCGCTCTCTG ACCTGCTGAA AGGGATGCTT GAGTACGAAC CGGCCAAGAG GTTCTCCATC
901      CGGCAGATCC GGCAGCACAG CTGGTTCCGG AAGAAACATC CTCCGGCTGA AGCACCAGTG
961      CCCATCCCAC CGAGCCCAGA CACCAAGGAC CGGTGGCGCA GCATGACTGT GGTGCCGTAC
1021     TTGGAGGACC TGCACGGCGC GGACGAGGAC GAGGACCTCT TCGACATCGA GGATGACATC
1081     ATCTACACTC AGGACTTCAC GGTGCCCGGA CAGGTCCCAG AAGAGGAGGC CAGTCACAAT
1141     GGACAGCGCC GGGGCCTCCC CAAGGCCGTG TGTATGAACG GCACAGAGGC GGCGCAGCTG
1201     AGCACCAAAT CCAGGGCGGA GGGCCGGGCC CCCAACCCTG CCCGCAAGGC CTGCTCCGCC
1261     AGCAGCAAGA TCCGCCGGCT GTCGGCCTGC AAGCAGCAGT GA

Quality Control: cDNA sequencing results is available upon request.