Human LIN28A Gene cDNA Clone / ORF Clone

LIN28A cDNA Clone Datasheet

LIN28A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_024674
• cDNA Size: 630
• Gene Synonym: CSDD1, LIN28, LIN-28, ZCCHC1, FLJ12457, LIN28A
• Source: Human

LIN28A cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens lin-28 homolog A (C. elegans) DNA.
• Vector: pGEM-T vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

LIN28A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LIN28A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LIN28A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTCCG TGTCCAACCA GCAGTTTGCA GGTGGCTGCG CCAAGGCGGC AGAAGAGGCG
61       CCCGAGGAGG CGCCGGAGGA CGCGGCCCGG GCGGCGGACG AGCCTCAGCT GCTGCACGGT
121      GCGGGCATCT GTAAGTGGTT CAACGTGCGC ATGGGGTTCG GCTTCCTGTC CATGACCGCC
181      CGCGCCGGGG TCGCGCTCGA CCCCCCAGTG GATGTCTTTG TGCACCAGAG TAAGCTGCAC
241      ATGGAAGGGT TCCGGAGCTT GAAGGAGGGT GAGGCAGTGG AGTTCACCTT TAAGAAGTCA
301      GCCAAGGGTC TGGAATCCAT CCGTGTCACC GGACCTGGTG GAGTATTCTG TATTGGGAGT
361      GAGAGGCGGC CAAAAGGAAA GAGCATGCAG AAGCGCAGAT CAAAAGGAGA CAGGTGCTAC
421      AACTGTGGAG GTCTAGATCA TCATGCCAAG GAATGCAAGC TGCCACCCCA GCCCAAGAAG
481      TGCCACTTCT GCCAGAGCAT CAGCCATATG GTAGCCTCAT GTCCGCTGAA GGCCCAGCAG
541      GGCCCTAGTG CACAGGGAAA GCCAACCTAC TTTCGAGAGG AAGAAGAAGA AATCCACAGC
601      CCTACCCTGC TCCCGGAGGC ACAGAATTGA

 

Quality Control:cDNA sequencing results is available upon request.