Mouse LIMK2 Gene cDNA Clone / ORF Clone

LIMK2 cDNA Clone Datasheet

LIMK2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010718.3
• cDNA Size: 1917
• Gene Synonym: C85310; Limk2a; Limk2b; 9430059K01; A930024P04Rik; Limk2
• Source: Mouse

LIMK2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus LIM motif-containing protein kinase 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 625 G/T resulting in the amino acid Gly substitution by Trp.

LIMK2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LIMK2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LIMK2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCGC TGGCGGGTGA TGAAGCCTGG AGGTGTCGGG GCTGTGGCAC CTATGTTCCC
61       CTGAGCCAGA GGCTCTATAG GACTGCCAAT GAAGCCTGGC ATGGCTCCTG CTTCCGGTGT
121      TCCGAATGCC AGGAATCCCT CACCAACTGG TACTATGAGA AGGATGGGAA GCTGTACTGC
181      CACAAGGACT ACTGGGCCAA GTTTGGAGAA TTCTGCCATG GGTGCTCCCT GCTGATGACG
241      GGGCCAGCCA TGGTGGCTGG GGAGTTTAAG TACCACCCAG AGTGCTTTGC CTGCATGAGT
301      TGCAAGGTGA TCATTGAGGA TGGAGATGCA TATGCCCTGG TGCAGCACGC TACACTCTAC
361      TGTGGGAAGT GCCACAACGA GGTGGTGCTG GCACCCATGT TTGAAAGACT GTCTACAGAG
421      TCTGTCCAGG ATCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCTGC TACCACCGAG
481      TGTAGACGCG GCTTCTCGGT GACTGTGGAA AGTGCCTCTT CTAACTATGC CACCACCGTT
541      CAAGTGAAAG AGGTCAACCG GATGCACATC AGTCCCAACA ACCGAAATGC CATCCACCCT
601      GGGGACCGCA TCTTGGAGAT CAATTGGACC CCTGTCCGTA CTCTGCGAGT AGAGGAGGTG
661      GAGGATGCAA TAAAGCAGAC AAGCCAGACA CTTCAGCTGC TGATTGAACA TGACCCTGTG
721      CCCCAGCGCC TGGACCAGCT ACGGCTAGAT GCCCGACTTC CTCCCCACAT GCAGAGCACC
781      GGACACACGC TCATGCTCAG CACCTTGGAC ACCAAGGAGA ATCAGGAGGG GACACTGAGG
841      AGACGTTCTC TGAGGCGCAG TAACAGCATC TCCAAGTCTC CGGGCCCCAG CTCCCCCAAG
901      GAGCCCCTGC TCCTCAGCCG GGACATAAGC CGCTCAGAAT CCCTCCGCTG CTCTAGCAGC
961      TACTCCCAGC AGATCTTCCG GCCCTGCGAC CTGATCCACG GGGAGGTCCT GGGGAAGGGC
1021     TTCTTTGGGC AGGCCATCAA GGTGACTCAC AAAGCCACAG GCAAAGTGAT GGTCATGAAG
1081     GAGTTGATCC GCTGTGATGA GGAAACCCAG AAGACTTTCC TGACTGAGGT GAAAGTGATG
1141     CGGAGCCTGG ACCACCCTAA TGTGCTCAAG TTCATCGGAG TTCTGTACAA GGACAAGAAG
1201     CTGAATCTGC TGACCGAGTA CATCGAGGGA GGCACACTCA AAGACTTCCT GCGCAGTGTG
1261     GACCCGTTCC CCTGGCAACA GAAGGTCAGG TTTGCCAAAG GCATCTCCTC TGGAATGGCC
1321     TATTTACACT CCATGTGCAT CATCCACCGG GACCTGAACT CACACAACTG TCTCATCAAA
1381     TTGGACAAGA CAGTGGTAGT AGCCGACTTT GGGCTGTCAC GGCTTATAGT AGAAGAGAGG
1441     AAAAGGCCTC CAGTAGAGAA GGCCACCACC AAGAAACGCA CCTTACGCAA GAGTGACCGC
1501     AAAAAGCGCT ACACTGTGGT GGGAAACCCC TACTGGATGG CCCCCGAGAT GCTGAATGGC
1561     AAGAGCTACG ATGAGACGGT GGATGTCTTC TCTTTTGGGA TCGTTCTCTG TGAGATCATT
1621     GGGCAGGTAT ATGCGGATCC TGATTGCCTG CCCCGCACAC TGGACTTTGG CCTCAATGTT
1681     AAGCTTTTCT GGGAGAAGTT TGTCCCAACA GACTGTCCCC CAGCCTTCTT CCCCCTGGCT
1741     GCTATCTGCT GCAAACTAGA GCCTGAAAGC AGACCGGCAT TCTCAAAGCT GGAGGACTCG
1801     TTCGAGGCGC TCTCCCTGTT CTTGGGGGAG CTGGCCATCC CACTGCCAGC AGAGCTGGAA
1861     GACCTGGACC ACACTGTGAG CATGGAGTAT GGCCTAACCC GGGACTCGCC ACCCTAG

 

Quality Control:cDNA sequencing results is available upon request.