Rat LILRA5 Protein

Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 Protein Datasheet

LILRA5 Protein Product Information

• Synonym: LILRA5
• Protein Construction: A DNA sequence encoding the rat LILRA5 (D4A6Y0)(Met1-Asn248) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

LILRA5 Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 17
• Molecular Mass: The recombinant rat LILRA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 473 amino acids and has a predicted molecular mass of 52.8 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LILRA5 protein

LILRA5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LILRA5 Protein Description

LILRA5, belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. They are also known as CD85, ILTs and LIR, and can exert immunomodulatory effects on a wide range of immune cells. ILT-11 contains 2 Ig-like C2-type (immunoglobulin-like) domains. It can be detected n tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.

References

1. Wende H. et al., 2000, Immunogenetics. 51 (8-9): 703-13.
2. Jones DC. et al., 2009, Eur J Immunol. 39 (11): 3195-206.
3. Mosbruger TL. et al., 2010, J Infect Dis. 201 (9): 1371-80.