Cynomolgus monkey LILRA4 Gene cDNA Clone / ORF Clone

LILRA4 cDNA Clone Datasheet

LILRA4 cDNA Clone Product Information

• cDNA Size: 1500
• Gene Synonym: LILRA4
• Source: Cynomolgus monkey

LILRA4 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1457 T/A, 171 C/G, 242 T/C resulting in the amino acid Val substitution by Asp, Asp substitution by Glu, Val substitution by Ala and 276 C/T, 486 T/C, 558 T/C not causing the amino acid variation.
  Please check the sequence information before order.

LILRA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LILRA4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LILRA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCCCCA TTCTCACAAC CCTGCTCTGC TTTGGGCTGA ACCTGGGCCC CAGGACCTGC
61       CTGCAGGCAG AGAACCTACG CAAACCCATC CTGTGGGCCG AGCCAGGTCC CGTGATCATC
121      TGGAAAAAGC CCGTGACCAT CTGGTGTCAG GGCACCCTGG AGGCCCAGGA GTACCGTCTG
181      GATAAAGAGG GAAACTCAAT GTTGAGGCAC ATGTTAAAAA CACTGGAGTC TGAAAACAAG
241      GCCAAATTCT CCATCCCATC CATGATGTGG GAACATGCAG GGCGATACCA CTGTTACTAT
301      CAGAGCCCTG CAGGCTGGTC AGAGCCCAGC GACCCCCTGG AGCTGGTGGT GACAGCCTAC
361      AGCAGACCCA GCCTGTCCGC CCTGCCGAGC CCTGTGGTGA CCTCCGGAGT GAACGTGACC
421      CTCCGGTGTG CCTCACGGCT GGGACTGGGC AGGTTCACTC TGATCGAGGA AGGAGACCAC
481      AGGCTCTCCT GGACCCTGGA CTCACACCAA CACAACCATG GAAAGTTCCA GGCCCTGTTC
541      CCCGTGGGTC CCCTGACCTT CAGCAACAGG GGTACGTTCA GATGCTACGG CTATGAAAAC
601      AACACCCCGT ACGTGTGGTC GGAACCCAGT GACCCCCTGC AGCTACTGGT GTCAGGTGTG
661      TCTAGGAAGC CCTCCCTCCT GACCCTGCAG GGCCCTGTCG TGGCCCCCGG AGACAACCTG
721      ACCCTCCAGT GTGGCTCTGA TGTCGGCTAC ATCAGATACG CTCTGTACAA GGAGGGGGGC
781      GATGGCCTCC CCCAGCGCCC TGGACAGCAG TCCCAGGCTG GGCTCTCCCA GGCCAGCTTC
841      ACCCTGAACC CTGTGAGGGG CTCCCACGGG GGCCAGTACA GATGCTACGG TGCACACAAT
901      GTCTCCTCCA AGTGGTCGGC CCCCAGCGAC CCCCTGGACA TCCTGATCAC AGGACAGATC
961      CCTGACAGAC CCTCCCTCTC AGTGCAGCTG GGCCCCACGG TGGCCTCAGG AGAGAAGGTG
1021     ACCCTGTTGT GTCAGTCATG GGGTCCGATG TTCACTTTCC TTCTGGCCAA GGAGGGGGCA
1081     GCCCATCCCC CGCTGCGTCT AAGATCCACT TACAGAGCTC AGCAGTACCA GGCTGAATTC
1141     CCCATGAGTC CCGTGACCTC GGCCCACGCG GGGACCTACA GGTGCTACGG CTCACGCAGC
1201     TCCGACCCCT ACCTGCTGTC TCACTCCAGT GAGCCCCTGG AGCTCGTGGT TTCAGAAGCA
1261     ACTGAGACCC TCAATCCAGC ACAAAATAAG TCAGATTCCA AGACTGCCCC ACACCTCCAG
1321     GATTACACAG TGGAGAATCT CATCCGCATG GGTATAGCTG GCTTGGTCCT GGTGTTCCTT
1381     GGGATTCTGT TATTTGAGGC TCAGCAGAGC CAGAGAAGCC CCACAAGGTG CAGCCAGGAG
1441     GTGAACAGCA GAGAAGACAA TGCACCCTTC AGAGTGGTGG AGCCCTGGGA ACAGATCTGA

 

Quality Control:cDNA sequencing results is available upon request.