Cynomolgus monkey LIFR Gene cDNA Clone / ORF Clone (Cytokine)

LIFR cDNA Clone Datasheet

LIFR cDNA Clone Product Information

• cDNA Size: 3258
• Gene Synonym: LIFR
• Source: Cynomolgus monkey

LIFR cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) leukemia inhibitory factor receptor alpha DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1675 T/C resulting in the amino acid Tyr substitution by His.
  Please check the sequence information before order.

LIFR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LIFR cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LIFR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGATA TTTACGTGTG TTTGAAACGA GCATCCTGGA TGGTGGACAA TAAAAGAATG
61       AGGACTGCTT CAAATTTCCA GTGGCTCTTA TCAACATTTA TTCTTCTATA TCTAATGAAT
121      CAAGTAAATA GCCAGAAAAA GGGAGCTCCT CGTGATTTGA AGTGTGTAAC TAACAATTTG
181      CAAGTGTGGG ACTGTTATTG GAAAGCACCC TCTGGAACAG GCCGTGGTAC TGATTATGAA
241      GTTTGCATTG AAAACAGGTC CCATTCTTGT TATCAACTAG AGAAAACAAA TATTAAAATT
301      CCAGCTCTTT CACATGGTGA TTATGAAATA ACAATAAATT CTCTACATGA TTTTGGAAGT
361      TCTACGAGTA AATTCACACT AAATGAACAA AACGTTTCCT TAATTCCAGA TACTCCAGAG
421      ATCTTGAATT TGTCTGCTGA TTTCTCAACC TCTACATTAT ACCTAAAGTG GAATGACAGG
481      GGTTCAGTTT TTCCACACCA CTCAAATGTT ATCTGGGAAA TTAAAATTCT ACGTAAAGAG
541      AGTATGGAGC TCGTAAAATT AGTGACCCAC AACACAACTC TGAATGGCAA AGATACACTT
601      CATCACTGGA GTTGGGCCTC AGATATGCCC TTGGAGTGTG CCATTCATTT TGTGGAAATT
661      AGATGCTACA TTGACAGTCT TCATTTTTCT GGTCGCAAAG AGTGGAGTGA CTGGAGCCCT
721      GTGAAGAACA TTTCTTGGAT GCCTGATTCT CAGACTAAGG TTTTTCCTCA AGATAAAGTG
781      ATACTTGTAG GCTCAGACAT AACATTTTGT TGTATAAGTC AAGAAAAAGT GTTATCAGCA
841      CTGATTGGCC ATACAAACTG CCCCTTGATC CATCTTGATG GGGAAAATGT TGCAATCAAG
901      ATTCATAATA TTTCTGTTTC TGCAAGTAGT GGAACAACTG TTATTTTTGC TGGATATCCA
961      CCAGATACTC CTCAACAACT GAATTGTGAG ACACATGATT TAAAAGAAAT TCTATGTAGT
1021     TGGAATCCAG GAAGGGCGAC AGCATTGGTG GGCCCACGTG CTACAAGTTA CACTTTACTT
1081     GAAAGTTTTT CAGGAAAATA TGTTAGATTT AAAAGAGCTG AAGCACCTAC AAATGGAAGC
1141     TATCAATTAT TCTTTCAAAT GCTTCCAAAT CAAGAAATAT ATAATTTTAC TTTGAATGCC
1201     CACAATCCTC TGGGTCGATC AGAATCAACA ATTTTAGTTA ATATAACTGA AAAAGTTTAT
1261     CCCCATACTC CTACTTCATT CAAAGTGAAG GATATTAATT CAACAGCTGT TAAACTTTCT
1321     TGGCATTTAC CAGGCAACTT TGCAAAGATT AATTTTTTAT GTCAAATTGA AATTAAGAAA
1381     TCTAATACAG TACAAGAACA GCGGAATGTC ACAATCAAAG GAGTAGAAAA TTCAAGTTAT
1441     CTTGTTGCTC TGGACAAGTT AAATCCATAC ACTGTATATA CTTTTCGGAT TCGTTGTTCT
1501     ACTGAAACTT TCTGGAAATG GAGCAAATGG AGCAATAAAA AACAACATTT AACCACAGAA
1561     GCCATTCCTT CAAAGGGGCC TGACACTTGG AGAGAGTGGA GTTCTGATGG AAAAAATTTA
1621     ATAATCTATT GGAAGCCTTT ACCCATTAAT GAAGCTAATG GAAAAATACT TTCCCACAAT
1681     GTGTCATGTT CATCAGATGA GGAAACACAG TCCCTTTCTG AAATCCCTGA TCCTCAGCAC
1741     AAAGCAGAGA TACAGCTTGA TAAGAATGAC TACATCATCA GCGTGGTGGC TAAAAATTCT
1801     GTGGGCTCGT CACCACCTTC CAAAATAGCG AGTATGGAAA TTCCAAATGA TGATCTCAAA
1861     ATAGAGCAAG TTGTTGGGAT GGGAAAGGGC ATTCTCCTCA CCTGGCATTA CGACCCCAAC
1921     ATGACTTGTG ACTACGTCAT TAAGTGGTGT AACTCGTCTC GGTCGGAACC ATGCCTTATG
1981     GACTGGAGAA AAGTCCCCTC AAACAGCACT GAAACTGTAA TCGAATCTGA TCAGTTTCGA
2041     CCAGGTGTAA GATATAATTT TTTCCTGTAT GGGTGCAGAA ATCAAGGATA TCAATTATTA
2101     CGCTCCATGA TTGGATATAT AGAAGAATTG GCTCCCACTG TTGCACCAAA TTTTACTGTT
2161     GAGGATACTT CTGCAGATTC GATATTAGTA AAATGGGAAG ACATTCCTGT GGAAGAACTT
2221     AGAGGCTTTT TAAGAGGATA TTTGTTTTAC TTTGGAAAAG GAGAAAGAGA CACATCAAAG
2281     ATGAGGGTTT TAGAATCAGG TCGTTCTGAC ATAAAGGTTA AGAATATTAC TGACATATCC
2341     CAGAAGACAC TGAGAATTGC TGACCTTCAA GGTAAAACAA GTTACCACCT GGTCTTGCGA
2401     GCCTATACAG ATGGTGGAGT GGGCCCAGAG AAGAGTATGT ACGTGGTGAC AAAGGAAAAT
2461     TCTGTGGGAT TAATTATTGC CATTCTCATC CCAGTGGCAG TGGCTGTCAT TGTTGGAGTG
2521     GTGACAAGTA TCCTTTGTTA TCGGAAGCGA GAATGGATTA AAGAAACCTT CTACCCTGAT
2581     ATTCCAAATC CAGAAAACTG TAAAGCGTTA CAGTTTCAAA AGAGTGTCTG TGAGGGAAGC
2641     AGTGGTCTTA AGACATTGGA AATGAATCCT TGTACCCCAA ATAATGTTGA GGTTCTAGAA
2701     ACTCGATCAG CGTTTCCTAA AATAGAAGAT ACAGAAATAA TTTCGCCAGT AGCTGAGCGT
2761     CCTGAAGATC GCTCTGATGC AGAGCCTGAA AACCATGTGG TTGTGTCCTA TTGTCCACCC
2821     ATCATTGAGG AAGAAATACC AAACCCAGCA GCAGATGAAG CTGGAGGGAC TGCACAGGTC
2881     ATTTACATTG ATGTTCAGTC GATGTATCAG CCTCAAGCAA AACCAGAAGA AGAACAAGAA
2941     AATGACCCTG TAGGAGGGGC AGGCTATAAA CCACAGATGC ACCTCCCCGT TAATTCTACT
3001     GTGGAAGATA TAGCTGCAGA AGAGGACTTA GAGAAAACTG CGGGTTACAG ACCTCAGGCC
3061     AATGTAAATA CATGGAATTT AGTGTCTCCA GACTCTCCTA GATCCATAGA CAGCAACAGT
3121     GAGATTGTCT CATTTGGAAG TCCATGCTCC ATTAATTCCC GACAATTTTT GATTCCTCCT
3181     AAAGATGAAG ACTCTCCTAA ATCTAATGGA GGAGGGTGGT CCTTTACAAA CTTTTTTCAG
3241     AACAAACCAA ACGATTAA

 

Quality Control:cDNA sequencing results is available upon request.