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Rat LIFR Gene cDNA Clone / ORF Clone (Cytokine)
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80322-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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LIFR cDNA Clone Datasheet
LIFR cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_031048.1 |
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cDNA Size: |
3282 |
| Gene Synonym: |
Lifr |
| Source: | Rat |
LIFR cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus leukemia inhibitory factor receptor alpha DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutations: 886 A/C resulting in the amino acid Asn substitution by His and 1053 G/A not causing the amino acid variation. |
LIFR cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
LIFR cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
LIFR cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGAGCTT TCTCATGGTG GAGACAGCCA TCTTGGATGG CGGACAATAA AAGAGGGAGG 61 ATGACTCCAA GCCTGCCATG GCTCCTGTCG GCTCTGACTC TTCTGCATCT GATGATGCAC 121 GTGAATGGTC TTAAGAGAGG GGTACAACAA GATCTGAAAT GCACAACCAA CAATATGCGA 181 GTGTGGGACT GCTCATGGCC AGCACCCCTC GGGGTCAGCC CTGGAACTGT TAAAGACATT 241 TGCATTAAAG ACAGGCCCCA TTCCTGCCAT CGACTAGAGA CAACAAACGT TAAGATCCCA 301 GCTCTTTCAC CCGGTGATCA CGAAGTAACA ATAAATTACC AAAACGGCTT TCAGAGTAAA 361 TTCACACTGA ATGAAAAAGA TGTTTCTTTA GTTCCGGATA CCCCTGAGAT CTTGAGTTTG 421 TCTGCTGACT TCTCGACCTC CACATTACAA CTGAAGTGGA ATGACAAAGG GTCTGCTCTC 481 CCTTACCCCT CCAATGCCAC CTGGGAGGTT AAGGTTCTGC AGAATCCGAG GACGGAGCCC 541 GTAGCACTAG TGTCACTCAA CACAGTGCTG AGTGGTAAAG ATAAAGGTCA CCACTGGAAC 601 TGGACCTCAG AACTGCCCTT GCAGTGTGCC ACTCACTCTG TGAGCATTAG ATGGCACATT 661 GACTACCCTC GTTTCTCTGG GTACAAAGAG TGGAGTGAGT GGAGCCCGCT GAAGAACATT 721 TCCTGGACGC GTAATACCGA GACTAACGTT TTTCCTCAAG ACAAAGTGGT ACTTGCAGGC 781 TCAAACATGA CAATTTGTTG TATAAGTACA ACGAAGGTGC TTTCAGGACA GATTGGAAAT 841 ACGTTCCGCC CTCTTATCCA TCTTTATGGG GAGACTGTCG CCATCCACAT CCTGAACATA 901 CCTGTTTCTG AAAACAGCGG CTCAAATGTG ATTTTCAGCA CAGTCGACGA TGTGTATGGA 961 ACGGTCGTCT TCGCAGGCTA TCCACCCGAT GTTCCTCAGA AACTGAGCTG TGAGACACAT 1021 GACTTGAAAG AGATTATATG TAGCTGGAAT CCAGGAAGGA TAACGGGACT GGTGGGCCCA 1081 CGCAACACAG AATACACCTT ATTTGAAAGC ATTTCAGGAA AATCGGCAGT GTTCCACAGG 1141 TTTGAAGAAC TTGCAAATGA AACCTATTGG TTAACCTTGA AAATGGCTCC TGACCAAGAA 1201 ATTCATAACT TCACCCTGAC TGCTCGCAAT CCACTGGGCC AGACAGAATC AGCGATAGTC 1261 ATCAACGCAA CTGAAAGAGT TGCTCTTCAT GTTCCTATTT CATTGAAAGT GAAGGACGTC 1321 AATTCAACAG TCGTTACACT TTCTTGGTAC TTGCCAGGAA ATTTTACAAA GATTAATCTT 1381 GTATGTCAGA TTGAAATTTG TAAAGCTAAT TCCAAGAAAG AAGTGCGGAA TGTCACAATG 1441 AGAGGAGCTG AGGATTCAAC CTACCATGTT GCTGTGGACA AACTAAACCC ATATACTATC 1501 TATACTTTCC GGGTTCGTTG TTCTTCCGAA ACTTTCTGGA AATGGAGCAA GTGGAGTAAT 1561 GAAAAGCGAT ATTTAACCAC TGAAGCCACT CCTTCAAAGG GACCAGATAC TTGGCGAGAG 1621 TGGAGTTCTG ATGGGAAAAA CTTAATCATC TACTGGAAGC CTTTACCCAT CAATGAAGCT 1681 AATGGAAAAA TACTTTCCTA CAATGTGTCC TGTTCATCAA GCGAGGAGAC ACAGTCCCTT 1741 TCCGAGATCC TCGATCCACA ACACAAAGCA GAGATAAAGG TTAATAAAAA TGACTACATC 1801 ATCAGTGTGG TGGCAAGAAA TTCTGCCGGT TCATCTCCAC CTTCAAAAAT AGCCAGTATG 1861 GAAATCCCAA ATGATGACAT CACGGTAGAG CAAGCGGTTG GAATAGGAAA CAGGATCTTC 1921 CTCAGCTGGC AGCACAACCC CAACATGACC TGTGACTACG TGATTAAATG GTGCAACTCG 1981 TCATGGTCTG AGCCCTGCCT CCTGGACTGG ATAAAGGTTC CCTCAAACAG CACGGGGACT 2041 GTCATCGAGT CTGATCAGTT TCAGCCAGGA GTGAGGTATA ACTTTTACCT CTATGGGTGT 2101 ACTAACCAGG GATATCAACT GTTACGTTCT ACAATTGGAT ACATAGAAGA ATTAGCTCCA 2161 ATTGTCGCAC CAAACTTTAC TGTGGAAGAC ACATCTGCAG ATTCGATATT AGTGAAGTGG 2221 GACGACATCC CCGTGGAGGA GCTCCGAGGC TTCCTTCGAG GGTATTTATT CTACTTCCAG 2281 AAAGGAGAGA GAGACACACC CAAGACACGG AGCTTAGAGA CAAGTCACTC TGACATCAAG 2341 CTAAAGAATA TCACTGACAT ATCCCAGAAG ACACTGCGGA TTGCCGACCT TCAGGGTAAA 2401 ACCAGTTACC ACCTGGTCCT GCGGGCTTAT ACACATGGTG GACTAGGCCC AGAGAAGAGC 2461 ATGTTTGTGG TGACCAAGGA GAACTCCGTG GGATTGATTA TTGCCATCCT CATCCCAGTG 2521 GCTGTGGCTG TCATTGTTGG CGTGGTAACA AGCATCCTCT GCTATCGGAA GCGAGAATGG 2581 ATTAAAGAAA CATTCTACCC TGATATTCCC AATCCAGAAA ACTGTAAGGC GTTACAGTTT 2641 CAGAAGAGCG TCTGTGAGGG AAGCAACGCT CTTAAGACAT TGGAAATGAA TCCATGCACC 2701 CCAAATCACG TCGAAGTCCT GGAATCTCGG TCAATACCCC CTAAAATAGA AGACACAGAA 2761 ATAACGTCCC CAGTTTCTGA GCGTCCTGGC GAAAGCTCTG AGACAGACCC CGAGAACCAG 2821 GCGGCCGTGT CTTACTGCCC ACCCATCATT GAGGAGGAAA TAACAAACCC AGCAGCAGAT 2881 GAGGCGGGAG GGGCTTCCCA GGTCGTGTAC ATTGATGTGC AGTCCATGTA TCAGCCACAA 2941 GCCAAAGCAG AGGACGAGCA GGACACTGAC CCCGTGATGG TGGCAGGCTA TAAGCCACAG 3001 ATGCGCCTCC CCATTAACCC CACAGCGGAA GACACAACAG CAGAAGATGA AGCGGACAAG 3061 ACCGCAGGTT ACAGACCTCA GGCCAACGTA AATACTTGGA ATTTGGTCTC CCCAGATTCC 3121 CCAAGGTCCA CAGACAGCAA CAGTGAAGTT GTCTCATTTG GAAGTCCGTG CTCCATCAAT 3181 TCCAGGCAAT TTTTGATTCC TCCTAAGGAT GAAGACTCTC CTAAATCTAA TGGAGGAGGG 3241 TGGTCCTTTA CAAACTTCTT CCAGAACAAA CCAAATGACT AA
Quality Control:cDNA sequencing results is available upon request.
