Text Size:AAA

Rat LIFR Gene cDNA Clone (full-length ORF Clone)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
LIFRcDNA Clone Product Information
Gene Bank Ref.ID:NM_031048.1
cDNA Size:3282
cDNA Description:ORF Clone of Rattus norvegicus leukemia inhibitory factor receptor alpha DNA.
Gene Synonym:Lifr
Species:Rat
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 886 A/C resulting in the amino acid Asn substitution by His and 1053 G/A not causing the amino acid variation.
Shipping Carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Rat LIFR Gene cDNA Clone (full-length ORF Clone) on other vectors
IL-6 Family & Receptor Related Products
Product nameProduct name
Human IL6ST / gp130 / CD130 Protein (His & Fc Tag)Human IL6ST / gp130 / CD130 ProteinMouse IL-11 / interleukin 11 ProteinSus scrofa (pig) IL6 / IL-6 ProteinMouse LIF Protein (Fc Tag)Mouse LIF Protein (His Tag)Human CNTF Protein (His Tag)Human GM-CSF / CSF2 Protein (Fc Tag)Human G-CSF / CSF3 Protein (Fc Tag)Human NNT1 / CLCF1 / CLC Protein (Fc Tag)Canine IL11RA / IL-11RA / IL11Rα ProteinRat CNTF / Ciliary Neurotrophic Factor ProteinRat CNTFR / CNTFR-alpha Protein (His Tag)Rat GM-CSF / CSF2 Protein (Fc Tag)Rat GM-CSF / CSF2 Protein (His Tag)Rat gp130 / IL6ST / CD130 Protein (His & Fc Tag)Rat gp130 / IL6ST / CD130 Protein (His Tag)Human CNTFR / CNTFR-alpha Protein (His Tag)Mouse Oncostatin M / OSM Protein (His Tag)Human Leptin ProteinHuman LIF Protein (His Tag)Rat IL6 / Interleukin-6 ProteinHuman LIF Protein (Fc Tag)Mouse Oncostatin M / OSM ProteinMouse CNTF / Ciliary Neurotrophic Factor Protein (His Tag)Human GM-CSF / CSF2 ProteinMouse IL11RA / IL11Rα Protein (His Tag)Human GM-CSF / CSF2 Protein (His Tag)Human GM-CSF / CSF2 ProteinHuman Oncostatin M / OSM Protein (His Tag)Mouse IL6 / Interleukin-6 ProteinRat LIFR Protein (His Tag)Human LIFR / CD118 Protein (His Tag)Human G-CSFR / CD114 / CSF3R Protein (Fc Tag)Human G-CSFR / CD114 Protein (His Tag)Human G-CSFR / CD114 / CSF3R ProteinHuman G-CSF / CSF3 Protein (isoform b)Human Oncostatin M / OSM ProteinCynomolgus / Rhesus IL6 / Interleukin-6 ProteinHuman IL11RA / IL11Rα Protein (His Tag)Mouse IL6ST / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His Tag)Mouse IL-31 / IL31 Protein (His Tag)Rat IL-6R / CD126 Protein (ECD, His Tag)Canine IL11RA / IL-11RA / IL11Rα Protein (His Tag)Human IL6 / Interleukin-6 ProteinHuman IL-31 / IL31 Protein (His Tag)Human G-CSF / CSF3 Protein (isoform b)Human CSF2RA / GM-CSFR / CD116 Protein (His Tag)Human CSF2RA / GM-CSFR / CD116 Protein (Fc Tag)Mouse GM-CSF / CSF2 ProteinHuman Leptin Receptor / LEPR / CD295 Protein (His & Fc Tag)Human Leptin Receptor / LEPR / CD295 Protein (His Tag)Human LIF ProteinCynomolgus IL6ST / gp130 Protein (Fc Tag)Cynomolgus IL6ST / gp130 Protein (His Tag)Mouse IL6RA / CD126 Protein (His Tag)Mouse GM-CSF / CSF2 Protein (Fc Tag)Mouse GM-CSF / CSF2 Protein (His Tag)Rat IL-6R / CD126 Protein (Fc Tag, ECD)Human Interleukin-31 receptor A / IL31RA Protein (His Tag)Human IL11 / Interleukin 11 / IL-11 ProteinHuman Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD)Human OSMR / IL31RB Protein (His Tag)Human IL6R / CD126 Protein (His Tag)Mouse LIFR / CD118 Protein (His Tag)Mouse OSMR / IL-31RB Protein (His Tag)
Background

LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (cardiotrophin like cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1 and cardiotrophin like cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

References
  • Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848.
  • Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65.
  • Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643.
  • Timmermann, A. et al.,2002, Eur. J. Biochem. 269 (11): 2716-2726.
  • Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096.
  • Catalog:RG80322-G
    List Price: $295.00  (Save $150.00)
    Price:$145.00      [How to order]
    Availability5 business days
    Images
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"