LIFR / CD118 Antibody (Cytokine)

LIFR Antibody Datasheet

LIFR Antibody Product Information

• Immunogen: Recombinant Mouse LIFR protein (Catalog#50423-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 110
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LIFR (rM LIFR; Catalog#50423-M08H; NP_038612.1; Met 1-Ser 828).

LIFR Antibody Usage Guide

• Specificity: Mouse LIFR
• No cross-reactivity in ELISA with

  Human LIFR

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse LIFR in WB. Using a DAB detection system, the detection limit for Mouse LIFR is approximately 5 ng/lane under non-reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse LIFR. The detection limit for Mouse LIFR is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LIFR Antibody Background

Leukemia inhibitory factor receptor alpha (LIFR), also known as CD118, is the receptor for leukemia inhibitory factor (LIF), a pleiotropic cytokine affecting the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an α subunit that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Human LIFRα subunit is a 1097 aa type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain including two cytokine receptor domains and three fibronectin type III repeats, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.

References

1. Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848.
2. Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65.
3. Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643.
4. Timmermann, A. et al.,2002, Eur. J. Biochem. 269 (11): 2716-2726.
5. Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096.