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Leukemia inhibitory factor Protein Datasheet
LIF Protein Price Inquiry ( Available Sizes )
LIF Protein Product Information
|Protein Construction:||A DNA sequence encoding the extracellular domain of mouse LIF (P09056) (Met 1-Phe 203) was fused with a polyhistidine tag at the C-terminus.|
|Expression Host:||Human Cells|
LIF Protein QC Testing
|Purity:||> 85 % as determined by SDS-PAGE.||SDS-PAGE:
|Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.
The ED50 for this effect is typically 0.1-0.5 ng/ml
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Ser 24|
|Molecular Mass:||The secreted recombinant mouse LIF comprises 191 amino acids and has a calculated molecular mass of 21.3 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 35-45 kDa in SDS-PAGE under reducing conditions.|
|Formulation:||Lyophilized from sterile PBS , pH 7.4
LIF Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
LIF Protein Related Products & Topics
LIF Protein Description
- Salas EM, et al. (2011) Genes Cancer. 2(5):593-6.
- Chodorowska G, et al. (2004) Ann Univ Mariae Curie Sklodowska Med. 59(2):189-93.
- García-Campaa AM, et al. (2007) Electrophoresis. 28(1-2):208-32.