Mouse LIF Protein (His Tag) (Cytokine)

Leukemia inhibitory factor Protein Datasheet

LIF Protein Product Information

• Synonym: RP23-329P17.1
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse LIF (P09056) (Met 1-Phe 203) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

LIF Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.
  The ED₅₀ for this effect is typically 0.1-0.5 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 24
• Molecular Mass: The secreted recombinant mouse LIF comprises 191 amino acids and has a calculated molecular mass of 21.3 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 35-45 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LIF protein

LIF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LIF Protein Description

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It shows a wide range of biologic activities that include the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. LIF is considered as a direct transcriptional target of STAT5 in myeloid cell lines: STAT5 binds to LIF promoter, and LIF expression is increased after activation of the JAK2/STAT5 pathway. LIF expression is significantly increased in patients with chronic myeloproliferative neoplasms with and without activating mutations of the pathway, indicating that LIF might play an important role in STAT5-mediated oncogenesis.

References

1. Salas EM, et al. (2011) Genes Cancer. 2(5):593-6.
2. Chodorowska G, et al. (2004) Ann Univ Mariae Curie Sklodowska Med. 59(2):189-93.
3. García-Campaa AM, et al. (2007) Electrophoresis. 28(1-2):208-32.