Human LGI4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13592-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LGI4 cDNA Clone Datasheet

LGI4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_139284.2
cDNA Size:	1614
Gene Synonym:	LGIL3;LGI4
Source:	Human

LGI4 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens leucine-rich repeat LGI family, member 4 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 456 T/C not causing the amino acid variation.

LGI4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGI4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGI4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGGGG CAGGCATTCT GCTGCTGCTG CTGGCTGGGG CGGGGGTGGT GGTGGCCTGG
61       AGACCCCCAA AGGGAAAGTG TCCCCTGCGC TGCTCCTGCT CTAAAGACAG CGCCCTGTGT
121      GAGGGCTCCC CGGACCTGCC CGTCAGCTTC TCTCCGACCC TGCTGTCACT CTCACTCGTC
181      AGGACGGGAG TCACCCAGCT GAAGGCCGGC AGCTTCCTGA GAATTCCGTC TCTGCACCTG
241      CTCCTCTTCA CCTCCAACTC CTTCTCCGTG ATTGAGGACG ATGCATTTGC GGGCCTGTCC
301      CACCTGCAGT ACCTCTTCAT CGAGGACAAT GAGATTGGCT CCATCTCTAA GAATGCCCTC
361      AGAGGACTTC GCTCGCTTAC ACACCTAAGC CTGGCCAATA ACCATCTGGA GACCCTCCCC
421      AGATTCCTGT TCCGAGGCCT GGACACCCTT ACTCACGTGG ACCTCCGCGG GAACCCGTTC
481      CAGTGTGACT GCCGCGTCCT CTGGCTCCTG CAGTGGATGC CCACCGTGAA TGCCAGCGTG
541      GGGACCGGCG CCTGTGCGGG CCCCGCCTCC CTGAGCCACA TGCAGCTCCA CCACCTCGAC
601      CCCAAGACTT TCAAGTGCAG AGCCATAGAG CTGTCCTGGT TCCAGACGGT GGGGGAGTCG
661      GCACTGAGCG TAGAGCCCTT CTCCTACCAA GGGGAGCCTC ACATTGTGCT GGCACAGCCC
721      TTCGCCGGCC GCTGCCTGAT TCTCTCCTGG GACTACAGCC TGCAGCGCTT CCGGCCCGAG
781      GAAGAGCTGC CCGCGGCCTC CGTGGTGTCC TGCAAGCCAC TGGTGCTGGG CCCGAGCCTC
841      TTCGTGCTGG CTGCCCGCCT GTGGGGGGGC TCACAGCTGT GGGCCCGGCC CAGTCCCGGC
901      CTGCGCCTGG CCCCAACGCA GACCCTGGCC CCGCGGCGGC TGCTGCGGCC CAATGACGCC
961      GAGCTCCTGT GGCTGGAAGG GCAACCCTGC TTCGTGGTGG CCGATGCCTC CAAGGCGGGC
1021     AGCACCACGC TGCTGTGCCG CGACGGGCCC GGCTTTTACC CGCACCAGAG CCTGCACGCC
1081     TGGCACCGGG ACACGGACGC TGAGGCCCTG GAGCTGGACG GCCGGCCCCA CCTGCTGCTG
1141     GCCTCGGCTT CCCAGCGGCC CGTGCTCTTC CACTGGACCG GTGGCCGCTT CGAGAGACGC
1201     ACAGACATCC CCGAGGCCGA GGATGTCTAT GCCACACGCC ACTTCCAGGC TGGTGGGGAC
1261     GTGTTCCTGT GCCTCACACG CTACATTGGG GACTCCATGG TCATGCGCTG GGACGGCTCC
1321     ATGTTTCGTC TGCTGCAGCA ACTTCCCTCG CGCGGTGCCC ACGTCTTCCA GCCACTGCTC
1381     ATCGCCAGGG ACCAGCTGGC CATCCTAGGC AGCGACTTCG CCTTCAGCCA GGTCCTCCGC
1441     CTTGAGCCTG ACAAGGGGCT CCTGGAGCCA CTGCAGGAGC TGGGGCCTCC GGCCCTGGTG
1501     GCCCCCCGTG CCTTTGCCCA CATCACTATG GCCGGCAGAC GCTTCCTCTT TGCTGCTTGC
1561     TTTAAGGGCC CCACACAGAT CTACCAGCAT CACGAGATCG ACCTCAGTGC CTGA

Quality Control:cDNA sequencing results is available upon request.