Human LGI1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13603-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LGI1 cDNA Clone Datasheet

LGI1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC022500
cDNA Size:	1674
Gene Synonym:	EPT;ETL1;IB1099;EPITEMPIN;LGI1
Source:	Human

LGI1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens leucine-rich, glioma inactivated 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

LGI1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGI1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGI1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT
61       TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
121      AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
181      ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
241      GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGTT ATTCACATCG
301      AACTCCTTTG ATGTGATCAG TGATGATGCT TTTATTGGTC TTCCACATCT AGAGTATTTA
361      TTCATAGAAA ACAACAACAT CAAGTCAATT TCAAGACATA CTTTCCGGGG ACTAAAGTCA
421      TTAATTCACT TGAGCCTTGC AAACAACAAT CTCCAGACAC TCCCAAAAGA TATTTTCAAA
481      GGCCTGGATT CTTTAACAAA TGTGGACCTG AGGGGTAATT CATTTAATTG TGACTGTAAA
541      CTGAAATGGC TAGTGGAATG GCTTGGCCAC ACCAATGCAA CTGTTGAAGA CATCTACTGC
601      GAAGGCCCCC CAGAATACAA GAAGCGCAAA ATCAATAGTC TCTCCTCGAA GGATTTCGAT
661      TGCATCATTA CAGAATTTGC AAAGTCTCAA GACCTGCCTT ATCAATCATT GTCCATAGAC
721      ACTTTTTCTT ATTTGAATGA TGAGTATGTA GTCATCGCTC AGCCTTTTAC TGGAAAATGC
781      ATTTTCCTTG AATGGGACCA TGTGGAAAAG ACCTTCCGGA ATTATGACAA CATTACAGGC
841      ACATCCACTG TAGTATGCAA GCCTATAGTC ATTGAAACTC AGCTCTATGT TATTGTGGCC
901      CAGCTGTTTG GTGGCTCTCA CATCTATAAG CGAGACAGTT TTGCAAATAA ATTCATAAAA
961      ATCCAGGATA TTGAAATTCT CAAAATCCGA AAACCCAATG ACATTGAAAC ATTCAAGATT
1021     GAAAACAACT GGTACTTTGT TGTTGCTGAC AGTTCAAAAG CTGGTTTTAC TACCATTTAC
1081     AAATGGAACG GAAACGGATT CTACTCCCAT CAATCCTTAC ACGCGTGGTA CAGGGACACT
1141     GATGTGGAAT ATCTAGAAAT AGTCAGAACA CCTCAGACAC TCAGAACGCC TCATTTAATT
1201     CTGTCTAGTA GTTCCCAGCG TCCTGTAATT TATCAGTGGA ACAAAGCAAC ACAATTATTC
1261     ACTAACCAAA CTGACATTCC TAACATGGAG GATGTGTACG CAGTGAAGCA CTTCTCAGTG
1321     AAAGGGGACG TGTACATTTG CTTGACAAGA TTCATTGGTG ATTCCAAAGT CATGAAATGG
1381     GGAGGCTCCT CGTTCCAGGA TATTCAGAGG ATGCCATCGC GAGGATCCAT GGTGTTCCAG
1441     CCTCTTCAAA TAAATAATTA CCAATATGCA ATTCTTGGAA GTGATTACTC CTTTACTCAA
1501     GTGTATAACT GGGATGCAGA GAAAGCCAAA TTTGTGAAAT TTCAGGAATT AAATGTTCAG
1561     GCACCAAGAT CATTCACACA TGTGTCCATT AATAAGCGTA ATTTTCTTTT TGCTTCCAGT
1621     TTTAAGGGAA ATACACAGAT TTACAAACAT GTCATAGTTG ACTTAAGCGC ATGA

Quality Control:cDNA sequencing results is available upon request.