Rat LGALS8 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80231-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LGALS8 cDNA Clone Datasheet

LGALS8 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_053862.2
cDNA Size:	951
Gene Synonym:	Lgals8
Source:	Rat

LGALS8 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus lectin, galactoside-binding, soluble, 8 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 239 T/C resulting in the amino acid Leu substitution by Pro.

LGALS8 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGALS8 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGALS8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGTCCT TAAGCAATCT ACAAAATATC ATCTATAACC CGACAATCCC CTATGTCAGT
61       ACCATTACTG AGCAGTTGAA GCCTGGCTCT TTGATCGTGA TCCGTGGCCA TGTTCCTAAA
121      GATTCAGAAA GATTCCAAGT AGACTTTCAG CATGGCAACA GCCTGAAGCC GAGAGCTGAT
181      GTGGCCTTCC ACTTTAACCC TCGCTTCAAA AGGTCCAACT GCATTGTTTG TAACACACCG
241      ACAAATGAGA AATGGGGCTG GGAGGAGATC ACCCACGACA TGCCTTTCAG AAAAGAAAAG
301      TCCTTTGAGA TTGTGATCAT GGTGCTAAAG AACAAATTCC AGGTGGCTGT GAATGGAAAG
361      CACATTCTGC TGTATGCCCA CAGGATCAAC CCAGAGAAGA TAGACACACT GGGCATCTAC
421      GGCAAAGTGA ACATTCACTC CATCGGGTTC AGATTCAGCT CGGATTTACA GAGTATGGAA
481      ACATCTACTC TGGGACTGAC ACAGATAAGT AAAGAAAATA TACAAAAGTC TGGCAAGCTC
541      CATTTGAGCC TGCCATTTGA AGCAAGGTTG AATGCCTCCA TGGGCCCTGG ACGAACCGTT
601      GTCGTTAAAG GAGAAGTGAA TACAAATGCC AAAAGCTTTA ATGTTGACCT AGTGGCAGGA
661      AGGTCAAGGG ATATCGCTCT GCACTTGAAC CCACGCCTGA ATGTGAAAGC GTTTGTAAGA
721      AACTCCTTTC TTCAGGATGC CTGGGGAGAA GAGGAGAGAA ACATTACCTG CTTCCCATTT
781      AGTTCTGGGA TGTACTTTGA GATGATAATT TACTGTGATG TCCGAGAGTT CAAGGTTGCA
841      GTAAATGGTG TGCACAGCCT GGAGTACAAG CACAGATTTA AAGACCTAAG CAGCATCGAC
901      ACACTAGCAG TTGATGGCGA TATCCGTTTG CTGGATGTAA GGAGCTGGTA G

Quality Control:cDNA sequencing results is available upon request.