LGALS8 / Galectin-8 Protein (Native)

Lectin, galactoside-binding, soluble, 8 (Galectin-8) Protein Datasheet

LGALS8 / Galectin-8 Protein Product Information

• Synonym: LGALS8,RP11-385F5.1, Gal-8, PCTA-1, PCTA1, Po66-CBP
• Protein Construction: A DNA sequence encoding human LGALS8 (AAF19370.1) (Met 1-Trp 317) was expressed and purified.
• Source: Human
• Expression Host: E.coli

LGALS8 / Galectin-8 Protein QC Testing

• Purity:	>97% as determined by SDS-PAGE.	SDS-PAGE: LGALS8 / Galectin-8 protein
  Bio-activity:	Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.8-4 μg/ml.
  Endotoxin:	Please contact us for more information.
  Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
  Predicted N terminal:	Met
  Molecular Mass:	The recombinant human LGALS8 consists of 316 amino acids and predicts a molecular mass of 35.5 kDa. The apparent molecular mass of rhLGALS8/GST is approximately 34 KDa in SDS-PAGE under reducing conditions.
  Formulation:	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

LGALS8 / Galectin-8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LGALS8 / Galectin-8 Protein Description

Galectin-8, also known as Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, PCTA-1 and LGALS8, is a member of the galectin family. Galectin-8 / LGALS8 was discovered in prostate cancer cells and has been studied extensively in the last few years. Various studies showed that galectin-8 is widely expressed in tumor tissues as well as in normal tissues. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. Galectin-8 / LGALS8 is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. The level of galectin-8 expression may correlate with the malignancy of human colon cancers and the degree of differentiation of lung squamous cell carcinomas and neuro-endocrine tumors. The differences in Galectin-8 / LGALS8 expression levels between normal and tumor tissues have been used as a guide for the selection of strategies for the prevention and treatment of lung squamous cell carcinoma. Galectin-4, Galectin-8 represents a subfamily of galectins consisting of a tandem repeat of structurally different carbohydrate recognition domains within a single polypeptide chain.

References

1. Hadari YR. et al., 1995, J Biol Chem. 270 (7): 3447-53.
2. Bidon N. et al., 2001, Int J Mol Med. 8 (3): 245-50.
3. Nishi N. et al., 2003, Glycobiology. 13 (11): 7 55-63.
4. Bidon-Wagner N. et al., 2004, Glycoconj J. 19 (7-9): 557-63.