LGALS7 / Galectin-7 Protein (GST Tag)

Lectin, galactoside-binding, soluble, 7 Protein Datasheet

LGALS7 / Galectin-7 Protein Product Information

• Synonym: GAL7, LGALS7A
• Protein Construction: A DNA sequence encoding the human LGALS7 (P47929) (Ser 2-Phe 136) was fused with the GST tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

LGALS7 / Galectin-7 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to agglutinate human red blood cells.
  The ED₅₀ for this effect is typically 0.01-0.05 μg/mL.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human LGALS7/GST chimera consists of 366 amino acids and has a predicted molecular mass of 41.8 kDa. It migrates as an approxiamtely 40 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LGALS7 / Galectin-7 protein

LGALS7 / Galectin-7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LGALS7 / Galectin-7 Protein Description

LGALS7, also known as Galectin-7, belongs to the galectins family. Galectins share similarities in the CRD (the carbohydrate recognition domain). LGALS7 belongs to the prototypical Galectins containing a single CRD, which is initially identified in human epidermis as a monomer. LGALS7 is specifically expressed in keratinocytes. It can be detected at all stages of epidermal differentiation. It is moderately repressed by retinoic acid. LGALS7 was found mainly in stratified squamous epithelium. It plays a role in cell–cell and/or cell–matrix interactions necessary for normal growth control. LGALS7 exhibits a pro-apoptotic function, as a new mitochondrial BCL2 interacting partner. It could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. LGALS7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

References

1. Villeneuve C. et al., 2011, Mol Biol Cell. 22 (7): 999-1013.
2. Rondanino C. et al., 2011, Am J Physiol Renal Physiol. 301 (3): F622-33.
3. Masuyer G. et al., 2012, FEBS J. 279 (2): 193-202.
4. Magnaldo T. et al., 1995, Dev Biol. 168 (2): 259-71.
5. Madsen P. et al., 1995, J Biol Chem. 270 (11): 5823-29.