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Rat LGALS3 Gene cDNA Clone (full-length ORF Clone)
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Rat LGALS3 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80393-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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LGALS3 cDNA Clone Datasheet

 

LGALS3 cDNA Clone Product Information

Gene Bank Ref.ID: NM_031832.1

cDNA Size:

789

Gene Synonym:

gal-3; MGC105387; Lgals3

Source: Rat

LGALS3 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus lectin, galactoside-binding, soluble, 3 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 42T>C and 126G>A not causing the amino acid variation.

LGALS3 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGALS3 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGALS3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGACG GCTTCTCACT TAATGATGCC TTAGCTGGCT CCGGAAACCC AAACCCTCGA
61       GGATGGCCTG GTGCATGGGG GAACCAGCCT GGGGCAGGAG GCTACCCAGG GGCCTCCTAT
121      CCTGGAGCCT ACCCAGGACA GGCTCCTCCA GGGGGTTATC CTGGACAGGC TCCTCCTAGT
181      GCCTATCCGG GCCCAACTGG CCCTAGTGCT TATCCTGGCC CAACTGCCCC TGGAGCTTAT
241      CCTGGCCCAA CTGCCCCCGG AGCCTTCCCA GGGCAACCTG GGGGACCTGG AGCCTACCCC
301      AGTGCTCCTG GGGCCTACCC CAGTGCTCCT GGGGCCTATC CTGCTACTGG CCCCTTTGGT
361      GCCCCGACTG GACCACTGAC AGTGCCCTAC GATATGCCCT TGCCTGGAGG AGTCATGCCT
421      CGCATGCTGA TCACAATCAT AGGCACAGTG AAGCCCAACG CAAACAGTAT CACTCTGAAT
481      TTCAAGAAAG GGAACGACAT CGCCTTCCAC TTTAACCCCC GCTTCAATGA GAACAACAGA
541      AGAGTCATCG TGTGCAACAC GAAGCAGGAC AATAACTGGG GAAGGGAAGA AAGACAGTCA
601      GCTTTCCCCT TTGAGAGCGG CAAACCATTC AAAATACAGG TCCTGGTTGA AGCCGACCAC
661      TTCAAGGTTG CGGTCAATGA TGTTCATCTG TTGCAGTATA ACCATCGGAT GAAGAACCTC
721      AGGGAAATCA GCCAACTGGG GATCATTGGT GACATAACCC TCACCAGCGC TTCCCACGCC
781      ATGATCTAA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

 

Galectin-3/LGALS3 related areas, pathways, and other information

 

 

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"