Rat LGALS1 Gene cDNA Clone / ORF Clone

LGALS1 cDNA Clone Datasheet

LGALS1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019904.1
• cDNA Size: 408
• Gene Synonym: Lgals1
• Source: Rat

LGALS1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus lectin, galactoside-binding, soluble, 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

LGALS1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGALS1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGALS1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTGTG GTCTGGTCGC CAGCAACCTG AATCTCAAAC CTGGGGAATG TCTCAAAGTT
61       CGGGGAGAGC TGGCCCCGGA CGCCAAGAGC TTTGTGTTGA ACCTGGGGAA AGACAGCAAC
121      AACCTGTGCC TACACTTCAA CCCCCGCTTC AACGCCCACG GAGATGCCAA CACCATTGTG
181      TGTAACAGCA AGGACGATGG GACCTGGGGA ACAGAACAAC GGGAGACTGC CTTCCCTTTC
241      CAGCCTGGGA GCATCACGGA GGTGTGCATC ACCTTTGACC AGGCTGACCT GACCATCAAG
301      CTGCCAGACG GGCATGAATT CAAATTCCCC AACCGCCTCA ACATGGAGGC CATCAACTAC
361      ATGGCGGCGG ATGGTGACTT CAAGATTAAG TGTGTGGCCT TTGAGTGA

 

Quality Control:cDNA sequencing results is available upon request.