Rat LGALS1 / Galectin-1 Protein (Native)

Lectin, galactoside-binding, soluble, 1 Protein Datasheet

LGALS1 / Galectin-1 Protein Product Information

• Synonym: Galectin1, Lgals1
• Protein Construction: A DNA sequence encoding the rat Galectin1 (P11762) (Ala2-Glu135) was expressed and purified.
• Source: Rat
• Expression Host: E.coli

LGALS1 / Galectin-1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 2
• Molecular Mass: The recombinant rat Galectin1 consists of 135 amino acids and has a predicted molecular mass of 14.9 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LGALS1 / Galectin-1 protein

LGALS1 / Galectin-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LGALS1 / Galectin-1 Protein Description

Galectin-1 (Gal-1, GAL1), the first identified member of the β-galactoside-binding animal lectin family, and exists as a homodimer of a single carbohydrate-recognition domain. Galectin-1 is expressed in a variety of cell types, including thymic epithelial cells, endothelial cells, dendritic cells, macrophages, fibroblasts, and bone marrow stromal cells. There is increased galectin-1 expression in many types of cancer, including colon, breast, ovary and prostate carcinomas. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. It is revealed that Galectin-1 can induce apoptosis of activated human T cells and human T leukaemia cell lines, while resting T cells also bound galectin-1, but did not undergo apoptosis. Galectin-1 modulates inflammatory responses and contributes to tumor progression and immune cell homeostasis. Galectin-1 regulates the inflammatory responses of neutrophils, mast cells, and macrophages and also associates with components of the complement system. Galectin-1 is thus suggested to act as an autocrine negative regulatory “cytokine,” killing T cells to terminate an immune response.

References

1. Nozomu Nishi. et al., 2008, Glycobiology. 18 (12): 1065-73.
2. Jiale He. et al., 2004, The Journal of Biological Chemistry. 279 (6): 4705-12.
3. Santiago Di Lella. et al., 2009, Biochemistry. 48 (4): 786-91.
4. Nicola TINARI1. et al., 2001, Int J Cancer: 91: 167-72.
5. Magali Fouillit. et al., 2000, Glycobiology. 10 (4): 413-9.