Human LFNG Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13494-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LFNG cDNA Clone Datasheet

LFNG cDNA Clone Product Information

Gene Bank Ref.ID:	BC014851
cDNA Size:	753
Gene Synonym:	SCDO3; LFNG
Source:	Human

LFNG cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminylt DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

LFNG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LFNG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LFNG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACACCAG GTCGCTGCTG TCTTGCGGCT GACATTCAGG TAGAGACGTT CATCTTCACT
61       GACGGGGAAG ATGAGGCCCT GGCCAGGCAC ACGGGCAACG TGGTCATCAC AAACTGCTCG
121      GCCGCCCACA GCCGCCAGGC GCTGTCCTGC AAGATGGCCG TGGAGTATGA CCGCTTCATC
181      GAGTCCGGCA GGAAGTGGTT CTGCCACGTG GACGATGACA ACTACGTCAA CCTGCGGGCC
241      CTGCTGCGGC TGCTGGCCAG CTACCCGCAC ACGCGGGACG TCTACGTCGG CAAGCCCAGC
301      CTGGACAGGC CCATCCAGGC CATGGAGCGG GTCAGCGAGA ACAAGGTGCG TCCTGTCCAC
361      TTCTGGTTTG CCACGGGCGG CGCTGGCTTC TGCATCAGCC GTGGGCTGGC TCTGAAGATG
421      AGCCCGTGGG CCAGCGGGGG TCACTTCATG AATACGGCTG AGCGGATCCG GCTGCCTGAT
481      GACTGCACCA TCGGCTACAT CGTGGAGGCC CTGCTGGGTG TGCCCCTCAT CCGCAGCGGC
541      CTCTTCCACT CCCACCTGGA GAACCTGCAG CAGGTGCCCA CCTCGGAGCT CCACGAGCAG
601      GTGACGCTGA GCTACGGTAT GTTTGAAAAC AAGCGGAACG CCGTCCACGT GAAGGGGCCC
661      TTCTCGGTGG AGGCCGACCC ATCCAGGTTC CGCTCCATCC ACTGCCACCT GTACCCGGAC
721      ACACCCTGGT GTCCCCGCAC TGCCATCTTC TAG

Quality Control:cDNA sequencing results is available upon request.