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LDLR / LDL Receptor Antibody, Rabbit MAb

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Mouse LDLR Antibody Product Information
Immunogen:Recombinant Mouse LDLR protein (Catalog#50305-M08H)
Clone ID:032
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR / LDL Receptor (rM LDLR; Catalog#50305-M08H; Met 1-Arg 790; NP_034830.2).
Mouse LDLR Antibody Usage Guide
Specificity:Mouse LDLR / LDL Receptor
No cross-reactivity in ELISA with
Human LDLR / LDL Receptor
Application:ELISA, ICC/IF, IF, FCM

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse LDLR. The detection limit for Mouse LDLR is approximately 0.00245 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 5-20 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Mouse LDLR Antibody IHC Application Image
LDLR / LDL Receptor Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Mouse LDLR in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Mouse LDLR Antibody FC Application Image
LDLR / LDL R / LDL Receptor Antibody, Rabbit MAb, Flow Cytometry
[Click to enlarge image]
Caption:
Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other LDLR Antibody Products
Reactivity: Human  
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            Reactivity: Human  
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            Reactivity: Mouse  
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              Reactivity: Mouse  
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                50305-RP02-50
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                Reactivity: Mouse  
                Application: FCM  
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                50305-R032-C-50
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                Reactivity: Mouse  
                Application: FCM  
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                50305-R032-F-50
                50305-R032-F-100
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                Reactivity: Mouse  
                Application: FCM  
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                50305-R032-P-50
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                Reactivity: Mouse  
                Application: ELISA  IHC-P  
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                LDLR/LDL R/LDL Receptor Background

                LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

                Mouse LDLR/LDL R/LDL Receptor References
              • Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
              • Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
              • Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
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                Catalog: 50305-R032-50
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                All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"