LDLR / LDL Receptor Antibody

LDLR / LDL Receptor Antibody

LDLR / LDL Receptor Antibody Product Information

• Immunogen: Recombinant human LDLR protein ( Catalog#10231-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 101
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human LDLR / LDL Receptor ( rh LDLR ; Catalog#10231-H08H ; NP_000518.1 ; Met 1 - Arg 788 ).

LDLR / LDL Receptor Antibody Usage Guide

• Specificity: Human LDLR / LDL Receptor
• No cross-reactivity in ELISA with
  Mouse LDLR
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human LDLR in WB. Using a DAB detection system, the detection limit for human LDLR is approximately 2 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human LDLR. The detection limit for human LDLR is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LDLR / LDL Receptor Antibody Background

Low-density lipoprotein (LDL) is a type of lipoprotein that transports cholesterol and triglycerides from the liver to peripheral tissues, and enables fats and cholesterol to move within the water-based solution of the blood stream. The Low-Density Lipoprotein Receptor (LDLR) is the prototype member of the LDLR family of scavenger receptors which is involved in receptor-mediated endocytosis of specific ligands. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. LDLR recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles, as well as the apoE protein found in chylomicron remnants and VLDL remnants (IDL). After taken into the cell ending up in lysosomes, LDL is degraded and the cholesterol is made available for repression of microsomal enzyme HMG CoA reductase, the rate-limiting step in cholesterol synthesis. As a mosaic protein, the extracellular region of LDLR consists of seven N-terminal complement-like cysteinerich repeats followed by three EGF like repeats with a β-propeller YWTD containing motif, and a membrane proximal Ser/Thr rich region. Furthermore, it is reported that in case of HIV-1 infection, LDLR functions as a receptor for extracellular Tat (HIV-1 transactivator) into neurons, mediating its internalization in uninfected cells. Mutations in LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia.

References

1. Suedhof, T.C. et al., 1985, Science. 228: 815-822.
2. Hobbs, H.H. et al., 1993, Hum. Mutat. 1: 445-466.
3. Barrett, P.H. and Watts, G.F. 2002, Atherosclerosis. Supplements. 2: 1-4.
4. Liu, Y. et al., 2000, Nat. Med. 6: 1380-1387.
5. Nomura, S. et al., 2004, Gene. Therapy. 11: 1540-1548.