LCN2 / NGAL Antibody

LCN2 / NGAL Antibody Datasheet

LCN2 / NGAL Antibody Product Information

• Immunogen: Recombinant Rat LCN2 / NGAL protein (Catalog#80058-R08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Rat LCN2 / NGAL (rR LCN2 / NGAL; Catalog#80058-R08B; P30152; Met 1-Asn 198). Total IgG was purified by Protein A affinity chromatography.

LCN2 / NGAL Antibody Usage Guide

• Specificity: Rat LCN2 / NGAL
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Rat LCN2 in WB. Using a DAB detection system, the detection limit for Rat LCN2 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat LCN2. The detection limit for Rat LCN2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LCN2 / NGAL Antibody Background

Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, a constituent of neutrophil granules, is a member of the lipocalin family of binding proteins. Mouse LCN2 gene was first isolated from mouse kidney, and is detected in lung, spleen, uterus, vagina and epididymis. LCN2 plays a role in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. It is indicted that NGAL exerts bacteriostatic effects, which are explained by its ability to capture and deplete siderophores, a small iron-binding molecules synthesized by certain bacteria as a means of iron acquisition. Further, acting as a growth and differentiation factor in multiple cell types, some of the activity of LCN2 is enhanced in the presence of siderophore: iron complexes.

References

1. Kjeldsen L. et al., 1993, J Biol Chem. 268 (14): 10425-32.
2. Goetz DH. et al., 2000, Biochemistry. 39 (8): 1935-41.
3. Schmidt-Ott KM. et al., 2007, J Am Soc Nephrol. 18 (2): 407-13.