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LCN2 / NGAL Antibody Datasheet
|Order or Inquire for LCN2 / NGAL Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.00975 ng/well in ELISA|
LCN2 / NGAL Antibody Product Information
Recombinant Rat LCN2 / NGAL protein (Catalog#80058-R08B)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Rat LCN2 / NGAL (rR LCN2 / NGAL; Catalog#80058-R08B; P30152; Met 1-Asn 198). Total IgG was purified by Protein A affinity chromatography.
LCN2 / NGAL Antibody Usage Guide
Rat LCN2 / NGAL
|Western blot :||This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Rat LCN2 in WB. Using a DAB detection system, the detection limit for Rat LCN2 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat LCN2. The detection limit for Rat LCN2 is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
LCN2 / NGAL Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|LCN2/NGAL||Human||LCN2/NGAL Protein, Recombinant||10222-H08H|
|LCN2/NGAL||Mouse||LCN2/NGAL Protein, Recombinant||50060-M08H|
|LCN2/NGAL||Rat||LCN2/NGAL Protein, Recombinant||80058-R08B|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||LCN2/NGAL Antibody, Rabbit PAb||10222-RP01|
|WB, ELISA||LCN2/NGAL Antibody, Rabbit Pab (Antigen Affinity Purified)||10222-RP02|
|WB, ELISA||Rabbit Polyclonal Antibody||50060-RP01|
|WB, ELISA||Rabbit Polyclonal Antibody (Antigen Affinity Purified)||50060-RP02|
|WB, ELISA||LCN2 / NGAL Antibody||50060-R211|
|WB, ELISA||LCN2 / NGAL Antibody||80058-RP01|
LCN2 / NGAL Antibody Background
Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, a constituent of neutrophil granules, is a member of the lipocalin family of binding proteins. Mouse LCN2 gene was first isolated from mouse kidney, and is detected in lung, spleen, uterus, vagina and epididymis. LCN2 plays a role in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. It is indicted that NGAL exerts bacteriostatic effects, which are explained by its ability to capture and deplete siderophores, a small iron-binding molecules synthesized by certain bacteria as a means of iron acquisition. Further, acting as a growth and differentiation factor in multiple cell types, some of the activity of LCN2 is enhanced in the presence of siderophore: iron complexes.
- Kjeldsen L. et al., 1993, J Biol Chem. 268 (14): 10425-32.
- Goetz DH. et al., 2000, Biochemistry. 39 (8): 1935-41.
- Schmidt-Ott KM. et al., 2007, J Am Soc Nephrol. 18 (2): 407-13.