Anti-LCN1 Antibody

Cat: 11583-RP02
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Anti-LCN1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-LCN1 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human LCN1
Immunogen
Recombinant Human LCN1 protein (Catalog#11583-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human LCN1 (rh LCN1; Catalog#11583-H08H; NP_002288.1; Met 1-Asp176). LCN1 specific IgG was purified by human LCN1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-LCN1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LCN1 Antibody (Rabbit Polyclonal antibody) Images
Human Lipocalin 1/LCN1 Western blot (WB) 13417

Anti-LCN1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:19 kDa

Observed band size:19 kDa

Anti-LCN1 Antibody Alternative Names
Anti-Lipocalin 1 Antibody;Anti-MGC71975 Antibody;Anti-PMFA Antibody;Anti-TLC Antibody;Anti-TP Antibody;Anti-VEGP Antibody
LCN1 Background Information

Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear prealbumin, VEGP, Tear lipocalin and LCN1, is a secreted protein which belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1 / VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1 / VEGP is identical with lingual von Ebner's gland protein, and is also produced in prostate, nasal mucosa and tracheal mucosa. Homologous proteins have been found in rat, pig and probably dog and horse. Lipocalin-1 / VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1 / VEGP acts as the principal lipid binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1 / VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1 / LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1 / LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.

Full Name
lipocalin 1
References
  • Lassagne H. et al., 1993, Exp. Eye Res. 56:605-609.
  • Redl,B. et al., 2000, Biochim Biophys Acta  1482 (1-2):241-8.
  • Wojnar P. et al., 2001, J. Biol. Chem. 276:20206-20212.
  • Wojnar P. et al., 2003, J. Biol. Chem. 278:16209-16215.
  • Breustedt D.A. et al., 2005, J. Biol. Chem. 280:484-493.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LCN1 Antibody Rabbit Polyclonal ELISA 11583-RP01
    Anti-LCN1 Antibody Mouse Monoclonal ELISA(Cap) 11583-MM06
    Anti-LCN1 Antibody Mouse Monoclonal ELISA,ELISA(Det) 11583-MM03
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