Human LCMT2 Gene cDNA Clone / ORF Clone

LCMT2 cDNA Clone Datasheet

LCMT2 cDNA Clone Product Information

• Gene Bank Ref.ID: BC015949
• cDNA Size: 2061
• Gene Synonym: PPM2; TYW4;LCMT2
• Source: Human

LCMT2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens leucine carboxyl methyltransferase 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

LCMT2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LCMT2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LCMT2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCCCCC GGAGCCGTGA GCGTCGGGCA GGCGCGGTAC AGAACACCAA CGACAGCAGC
61       GCCCTCAGCA AGCGTTCCCT GGCCGCGCGC GGGTACGTGC AGGACCCCTT TGCCGCGTTG
121      CTGGTTCCGG GCGCGGCGCG CCGCGCACCG CTCATTCACC GAGGCTACTA CGTCCGCGCA
181      CGCGCCGTGA GGCACTGCGT GCGCGCTTTT TTGGAGCAGA TTGGCGCGCC CCAGGCCGCG
241      CTTCGCGCGC AGATCTTGTC TCTCGGCGCT GGCTTCGACT CGCTCTATTT TCGCTTAAAA
301      ACCGCGGGCC GCCTGGCCCG GGCTGCAGTC TGGGAGGTGG ATTTTCCGGA CGTGGCGCGG
361      CGCAAAGCAG AAAGGATTGG AGAGACGCCA GAGCTGTGCG CGTTAACCGG GCCTTTCGAG
421      AGGGGGGAGC CCGCGTCCGC GCTGTGCTTT GAGAGCGCAG ACTACTGCAT CCTGGGTCTG
481      GACTTGCGGC AGCTCCAGCG AGTGGAGGAG GCCCTGGGCG CCGCGGGGCT CGACGCAGCC
541      TCACCCACTC TGCTCCTGGC CGAGGCGGTG CTGACCTACC TCGAGCCGGA GAGTGCCGCG
601      GCCCTCATCG CCTGGGCAGC CCAGCGTTTT CCTAATGCCC TTTTCGTGGT CTATGAGCAG
661      ATGAGGCCTC AAGACGCCTT TGGCCAGTTC ATGCTGCAAC ATTTTCGGCA GCTAAACTCC
721      CCCCTGCATG GCCTGGAGCG TTTTCCTGAC GTGGAGGCGC AGCGGCGCCG CTTCCTTCAA
781      GCTGGCTGGA CCGCCTGCGG TGCCGTGGAC ATGAATGAAT TCTATCACTG CTTTCTTCCC
841      GCAGAAGAAC GCCGGCGGGT GGAAAATATT GAACCCTTTG ACGAATTTGA GGAGTGGCAT
901      CTGAAGTGCG CCCATTATTT CATTCTGGCA GCTTCTAGGG GAGACACCCT CTCCCACACC
961      CTAGTGTTTC CATCCTCAGA GGCATTTCCT CGCGTAAATC CTGCTTCGCC TTCAGGGGTA
1021     TTCCCTGCCA GCGTAGTCAG TAGCGAGGGC CAGGTCCCAA ACCTGAAGAG ATATGGCCAC
1081     GCCTCTGTCT TCTTGAGCCC AGACGTTATT CTCAGTGCAG GAGGATTTGG AGAGCAGGAG
1141     GGGCGGCACT GCCGAGTGAG CCAGTTTCAC TTGCTCTCAA GAGATTGTGA CTCTGAATGG
1201     AAAGGCAGCC AAATAGGCAG TTGTGGGACT GGAGTTCAGT GGGATGGACG CCTTTATCAC
1261     ACCATGACAA GACTCTCAGA GAGTCGGGTT CTGGTTCTGG GAGGGAGACT GTCCCCAGTA
1321     AGTCCAGCCT TGGGGGTTCT CCAGCTTCAT TTTTTTAAGA GTGAGGATAA TAACACTGAG
1381     GACCTGAAAG TGACAATAAC AAAGGCTGGC CGAAAGGATG ATTCCACTTT GTGTTGTTGG
1441     CGGCATTCAA CAACAGAAGT GTCCTGTCAG AATCAGGAAT ATTTGTTTGT GTATGGGGGT
1501     CGAAGCGTGG TGGAACCTGT ACTAAGTGAC TGGCATTTCC TCCATGTAGG GACAATGGCT
1561     TGGGTCAGGA TCCCAGTGGA GGGAGAAGTA CCTGAAGCCC GGCATTCTCA CAGTGCCTGC
1621     ACTTGGCAAG GGGGAGCCCT TATTGCTGGA GGTCTCGGGG CTTCTGAGGA GCCATTGAAC
1681     TCTGTGCTCT TTCTGAGACC AATCTCTTGT GGATTCCTCT GGGAGTCAGT AGACATCCAG
1741     CCTCCCATTA CCCCAAGGTA CTCCCACACA GCTCATGTGC TCAATGGAAA GCTGTTACTG
1801     GTTGGAGGGA TCTGGATTCA TTCCTCCTCA TTTCCTGGAG TGACTGTGAT CAATTTGACT
1861     ACAGGATTGA GCTCTGAGTA TCAGATTGAC ACAACATATG TGCCATGGCC ATTAATGTTA
1921     CACAACCATA CTAGTATCCT TCTTCCTGAA GAGCAACAGC TCCTGCTCCT TGGAGGTGGT
1981     GGGAACTGCT TTTCCTTTGG TACCTACTTC AACCCCCATA CAGTCACATT AGACCTTTCT
2041     TCCTTAAGTG CTGGGCAGTA A

 

Quality Control:cDNA sequencing results is available upon request.