Human LCAT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13271-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LCAT cDNA Clone Datasheet

LCAT cDNA Clone Product Information

Gene Bank Ref.ID:	BC014781
cDNA Size:	1323
Gene Synonym:	LCAT
Source:	Human

LCAT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens lecithin-cholesterol acyltransferase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

LCAT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LCAT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LCAT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCCGC CCGGCTCCCC ATGGCAGTGG GTGACGCTGC TGCTGGGGCT GCTGCTCCCT
61       CCTGCCGCCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT
121      GAGCTCAGTA ACCACACACG GCCCGTCATC CTCGTGCCCG GCTGCCTGGG GAATCAGCTA
181      GAAGCCAAGC TGGACAAACC AGATGTGGTG AACTGGATGT GCTACCGCAA GACAGAGGAC
241      TTCTTCACCA TCTGGCTGGA TCTCAACATG TTCCTACCCC TTGGGGTAGA CTGCTGGATC
301      GATAACACCA GGGTTGTCTA CAACCGGAGC TCTGGGCTCG TGTCCAACGC CCCTGGTGTC
361      CAGATCCGCG TCCCTGGCTT TGGCAAGACC TACTCTGTGG AGTACCTGGA CAGCAGCAAG
421      CTGGCAGGGT ACCTGCACAC ACTGGTGCAG AACCTGGTCA ACAATGGCTA CGTGCGGGAC
481      GAGACTGTGC GCGCCGCCCC CTATGACTGG CGGCTGGAGC CCGGCCAGCA GGAGGAGTAC
541      TACCGCAAGC TCGCAGGGCT GGTGGAGGAG ATGCACGCTG CCTATGGGAA GCCTGTCTTC
601      CTCATTGGCC ACAGCCTCGG CTGTCTACAC TTGCTCTATT TCCTGCTGCG CCAGCCCCAG
661      GCCTGGAAGG ACCGCTTTAT TGATGGCTTC ATCTCTCTTG GGGCTCCCTG GGGTGGCTCC
721      ATCAAGCCCA TGCTGGTCTT GGCCTCAGGT GACAACCAGG GCATCCCCAT CATGTCCAGC
781      ATCAAGCTGA AAGAGGAGCA GCGCATAACC ACCACCTCCC CCTGGATGTT TCCCTCTCGC
841      ATGGCGTGGC CTGAGGACCA CGTGTTCATT TCCACACCCA GCTTCAACTA CACAGGCCGT
901      GACTTCCAAC GCTTCTTTGC AGACCTGCAC TTTGAGGAAG GCTGGTACAT GTGGCTGCAG
961      TCACGTGACC TCCTGGCAGG ACTCCCAGCA CCTGGTGTGG AAGTATACTG TCTTTACGGC
1021     GTGGGCCTGC CCACGCCCCG CACCTACATC TACGACCACG GCTTCCCCTA CACGGACCCT
1081     GTGGGTGTGC TCTATGAGGA TGGTGATGAC ACGGTGGCGA CCCGCAGCAC CGAGCTCTGT
1141     GGCCTGTGGC AGGGCCGCCA GCCACAGCCT GTGCACCTGC TGCCCCTGCA CGGGATACAG
1201     CATCTCAACA TGGTCTTCAG CAACCTGACC CTGGAGCACA TCAATGCCAT CCTGCTGGGT
1261     GCCTACCGCC AGGGTCCCCC TGCATCCCCG ACTGCCAGCC CAGAGCCCCC GCCTCCTGAA
1321     TAA

Quality Control:cDNA sequencing results is available upon request.